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A.M. Bron, N. Acar, C. Joffre, S. Grégoire, S. Almanza, S. Arnavielle, A. Guilaubey, C. Creuzot–Garcher, L. Bretillon; Docosahexaenoic Acid and Ether–lipid Levels Are Correlated to ERG Alterations During Retinal Ischemia–reperfusion in the Rat . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1614.
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Purpose: The retinal ischemia–reperfusion provides a relevant model of retinal oxidative stress. The purpose of this study was to evaluate the variations of retinal lipids known to be sensitive to oxidative stress –docosahexaenoic acid and ether lipids– after ischemia–reperfusion in rats. The relationships with retinal function were also studied. Methods: Retinal ischemia was induced on Wistar rats for 60 minutes by raising intraocular pressure to 110 mm Hg by canulation of the anterior chamber of one eye. The other eye was used as control. After 24 hours, 7 days and 14 days of reperfusion, the scotopic ERG was recorded on both eyes. Lipid were extracted from retinas and analyzed by gas chromatography. Results: The scotopic ERG a–wave and b–wave amplitudes were significantly reduced in treated eyes compared to control eyes. The b–wave amplitude showed a partial time–dependent recovery as judged by the reduction observed in treated eyes compared to controls: –82%, –58%, –51% after 24 hours, 7 days and 14 days of reperfusion, respectively. By contrast, a stable 50%–reduction of the a–wave amplitude was observed in the ischemic eye. These alterations in the visual function were correlated to a significant decrease in retinal docosahexaenoic acid levels (–16% after 14 days of reperfusion). Within ether–lipids, species linked to oleic acid were significantly reduced in treated eyes after 14 days of reperfusion. Conclusions: These results show that oxidative stress modifies the lipid content of the retina, docosahexaenoic acid –the most prominent fatty acid in photoreceptor outer segments– being the most altered. This study strongly suggests a link between retinal lipid levels and retinal function, as illustrated by the ERG a–wave amplitude. Based on the modification of the ether–lipid content, our results provide new data on their putative anti–oxidative properties roles within the retina.
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