Purchase this article with an account.
A.W. Siu, G.Y. Tang, A.K. Yip, R.Y. Chan; Glutamate Induced Retinal Lipid Peroxidation in vitro: Protective Effects of Pinoline and N–acetylserotonin . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1629.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: Glutamate is a neurotransmitter naturally found in the retina but excessive secretion of glutamate is reportedly related to retinal disorders, e.g. glaucoma and diabetic retinopathy. This study investigated the oxidative damage in the glutamate–treated retina and the protective effects of pinoline and N–acetylserotonin (NAS). Methods: Porcine retinal homogenates were mixed with 200 mM glutamate solution and co–incubated with various concentrations of pinoline, NAS or pinoline–NAS mixtures at 37°C for 60 min. The amounts of malondialdehyde (MDA) and protein were assayed to quantify the lipid peroxidation (LPO). Results: Both pinoline and NAS significantly suppressed the glutamate–induced retinal MDA in a dose–dependent manner (p<0.0001). Their effects were not significantly different from each other (p=0.51). Mixtures of pinoline and NAS demonstrated synergistic protection against glutamate–induced retinal MDA. Conclusions: Pinoline and NAS demonstrated anti–oxidative potency against glutamate–induced retinal LPO. Further investigation is required to understand the synergistic potential of pinoline and NAS in retinal patho–physiology.
This PDF is available to Subscribers Only