May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Glutamate Induced Retinal Lipid Peroxidation in vitro: Protective Effects of Pinoline and N–acetylserotonin
A.W. Siu; G.Y. Tang; A.K. Yip; R.Y. Chan
Author Affiliations & Notes
  • A.W. Siu
    Optometry, Hong Kong Polytechnic Univ, Hong Kong, Hong Kong Special Administrative Region of China
  • G.Y. Tang
    Optometry, Hong Kong Polytechnic Univ, Hong Kong, Hong Kong Special Administrative Region of China
  • A.K. Yip
    Optometry, Hong Kong Polytechnic Univ, Hong Kong, Hong Kong Special Administrative Region of China
  • R.Y. Chan
    Optometry, Hong Kong Polytechnic Univ, Hong Kong, Hong Kong Special Administrative Region of China
  • Footnotes
    Commercial Relationships  A.W. Siu, None; G.Y. Tang, None; A.K. Yip, None; R.Y. Chan, None.
  • Footnotes
    Support  PolyU grant G–T952
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1629. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Glutamate Induced Retinal Lipid Peroxidation in vitro: Protective Effects of Pinoline and N–acetylserotonin
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      A.W. Siu, G.Y. Tang, A.K. Yip, R.Y. Chan; Glutamate Induced Retinal Lipid Peroxidation in vitro: Protective Effects of Pinoline and N–acetylserotonin . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1629.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: Glutamate is a neurotransmitter naturally found in the retina but excessive secretion of glutamate is reportedly related to retinal disorders, e.g. glaucoma and diabetic retinopathy. This study investigated the oxidative damage in the glutamate–treated retina and the protective effects of pinoline and N–acetylserotonin (NAS). Methods: Porcine retinal homogenates were mixed with 200 mM glutamate solution and co–incubated with various concentrations of pinoline, NAS or pinoline–NAS mixtures at 37°C for 60 min. The amounts of malondialdehyde (MDA) and protein were assayed to quantify the lipid peroxidation (LPO). Results: Both pinoline and NAS significantly suppressed the glutamate–induced retinal MDA in a dose–dependent manner (p<0.0001). Their effects were not significantly different from each other (p=0.51). Mixtures of pinoline and NAS demonstrated synergistic protection against glutamate–induced retinal MDA. Conclusions: Pinoline and NAS demonstrated anti–oxidative potency against glutamate–induced retinal LPO. Further investigation is required to understand the synergistic potential of pinoline and NAS in retinal patho–physiology.

Keywords: retina • oxidation/oxidative or free radical damage 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept