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T. Sakai, N. Kuno, F. Takamatsu, H. Kohno, K. Kozaki, K. Kitahara; Prolonged Protective Effect of Basic Fibroblast Growth Factor–Loaded Microparticles in Royal College of Surgeons (RCS) Rats . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1679.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the protective effect of basic fibroblast growth factor–loaded microparticles (bFGF–MPs) against photoreceptor degeneration in Royal College of Surgeons (RCS) rats. Methods: Three week–old RCS rats received intravitreal injections of phosphate– buffered saline, free bFGF (2.5 µg) and bFGF–MPs (0.5 or 2.5 µg). Electroretinograms (ERGs) were recorded 4 and 8 weeks after injection. At the end of the experiment, retinas were prepared for light microscopy or immunocytochemical staining with antibodies to rod opsin, S and M cone opsins, and glial fibrillary acidic protein. Cell death was examined with the TUNEL method. Results: In the bFGF–MPs–treated eyes, the number of cones was larger and the number of TUNEL–positive cells was smaller than in the free bFGF–treated eyes 8 weeks after injection (p<0.05). Amplitudes of ERG b–waves 8 weeks after injection, however, did not differ between the free bFGF–treated eyes and the bFGF–MPs–treated eyes. Conclusions: These results suggest that bFGF–MPs have a prolonged protective effect against photoreceptor degeneration in RCS rats.
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