May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Ca2+–sensor Neurocalcin Delta Modulated Site in ROS–GC1–linked Signaling Pathway in Certain Ganglion Cells
Author Affiliations & Notes
  • V. Venkataraman
    Unit of Reg & Mol Biol Cell, UMNDJ, Stratford, NJ
  • T. Duda
    Unit of Reg & Mol Biol Cell, UMNDJ, Stratford, NJ
  • N. Vardi
    Neuroscience, University of Pennsylvania, Philadelphia, PA
  • E. Fik–Rymarkiewicz
    Unit of Reg & Mol Biol Cell, UMNDJ, Stratford, NJ
  • R.K. Sharma
    Unit of Reg & Mol Biol Cell, UMNDJ, Stratford, NJ
  • Footnotes
    Commercial Relationships  V. Venkataraman, None; T. Duda, None; N. Vardi, None; E. Fik–Rymarkiewicz, None; R.K. Sharma, None.
  • Footnotes
    Support  NIH EY 10828, DC 005349, HL 070015
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1732. doi:
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      V. Venkataraman, T. Duda, N. Vardi, E. Fik–Rymarkiewicz, R.K. Sharma; Ca2+–sensor Neurocalcin Delta Modulated Site in ROS–GC1–linked Signaling Pathway in Certain Ganglion Cells . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1732.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:Previous studies established the presence of the Ca2+–modulated neurocalcin delta/ROS–GC1 transduction machinery in the inner retinal neurons. The present study was designed to determine the neurocalcin delta–modulatory site in ROS–GC1 signaling pathway and localize the pathway in the inner retina. Methods:Mutagenesis/in vitro reconstitution experiments, western analyses, surface plasmon resonance spectroscopy and immunostaining. Results:In vitro reconstitution experiments were carried out to test the response of different deletion mutants of ROS–GC1 to neurocalcin delta. Deletion of aa 965–1054 had no effect on the Ca2+–dependent stimulation of the cyclase and the bacterially expressed ROS–GC1 fragment aa 965–1054 did not directly bind neurocalcin delta. Immunostaining analyses showed that ROS–GC1 and neurocalcin delta are present in certain ganglion cells. Thus, in response to Ca2+, neurocalcin delta binds to the specified domain of ROS–GC1 and activates the cyclase. Conclusions:In retina, ROS–GC1 is modulated by four Ca2+–sensor proteins: GCAP1, GCAP2, S100 beta and neurocalcin delta. Ca2+–dependent activation of ROS–GC1 by neurocalcin delta occurs via a unique cyclase domain – aa 733– 964. The pathway appears to be functional in a sub–population of large ganglion cells.

Keywords: calcium • ganglion cells 
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