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H. Okamoto, S. Umeda, M.T. Suzuki, K. Terao, Y. Yoshikawa, Y. Tanaka, T. Iwata; Comparative Proteome Analysis of Macula versus Peripheral Retina in Cynomolgus Monkey . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1757.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Age–related macular degeneration (AMD) is a leading disease for elderly which preferentially affects the macular region of the retina. This may be due to the tissue characteristic and proteins expressed in the macular region. To determine the proteins specifically expressed in the macular comprehensive proteome analysis of macular versus peripheral retina was performed by 2D gel separation and mass spectrometer. Methods: Tissue from macular and peripheral retina was punched in 3mm diameter from 2 normal monkeys and pooled to prepare protein extract. 2D gel electrophoresis was performed to separate proteins followed by gel scanning and spot comparison between macular and peripheral retina. Identification of protein in gel spot was carried out by ion spray LC–MS/MS (LCQ Deca XP plus, ThermoElectron). Results: Approximately two hundred spots were detected on the gel image for macular and periphery retina samples respectively. Seventy percent of these spots were present in both samples. Forty nine spots in the macular were not detected in the peripheral retina. These spots included X–arrestin and other proteins expressed in macular. Detail list of identified proteins will be shown at the meeting. Conclusions: Macular specific proteins were identified by 2D gel and mass spectrometer. Forty nine out of two hundred spots were identified as specific to macular. Among the identified proteins were X–arrestin and other cone specific proteins. These proteins are currently being investigated by immunohistochemical experiments.
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