May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Contact Lenses as a Diagnostic Tool II: Hormone Uptake by Poly–Electrolyte Coated Surfaces and Contact Lenses
Author Affiliations & Notes
  • P. Segu
    Tertc, University of Houston, Houston, TX
  • B. Hughes
    Tertc, University of Houston, Houston, TX
  • S. Narayanan
    Tertc, University of Houston, Houston, TX
  • F.P. Carney
    CIBA Vision, Duluth, GA
  • C. Morris
    Southern Cross University, Nsw, Australia
  • M. Venugopal
    CIBA Vision, Duluth, GA
  • L. Chapoy
    HPM, Barrington, IL
  • Y. Lvov
    LA Tech, Ruston, LA
  • A. McDermott
    Tertc, University of Houston, Houston, TX
  • J.P. Bergmanson
    Tertc, University of Houston, Houston, TX
  • Footnotes
    Commercial Relationships  P. Segu, CIBA Vision F; B. Hughes, CIBA Vision F; S. Narayanan, CIBA Vision F; F.P. Carney, CIBA Vision E; C. Morris, CIBA Vision E; M. Venugopal, CIBA Vision E; L. Chapoy, CIBA Vision F; Y. Lvov, CIBA Vision F; A. McDermott, CIBA Vision F; J.P. Bergmanson, CIBA Vision F.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 2073. doi:
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      P. Segu, B. Hughes, S. Narayanan, F.P. Carney, C. Morris, M. Venugopal, L. Chapoy, Y. Lvov, A. McDermott, J.P. Bergmanson; Contact Lenses as a Diagnostic Tool II: Hormone Uptake by Poly–Electrolyte Coated Surfaces and Contact Lenses . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2073.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Contact lenses may be useful collection devices for detecting various analytes in the tear film. With a view to determining their potential to assist in monitoring fertility, we have investigated if hormones are taken up by poly electrolyte coated slides and lenses. Methods: Glass slides were coated with different charged coatings prepared in the presence or absence of salt using layer–by–layer technology. The slides were incubated with either 3H–estradiol (2–3000pg/ml) or 3H–progesterone (5–100nM) diluted in phosphate buffered saline or artificial tear solution for 2 hours at room temperature with shaking. The slides were stored over night at 4oC, then the amount of hormone bound was determined by liquid scintillation counting. Nelfilcon A lenses with or without coatings were soaked in lutenizing hormone (0.1ug/ml – 0.5mg/ml) overnight, washed, and the amount of hormone bound was detected by enzyme immunoassay. Results: All coated slides showed higher hormone binding than control uncoated slides except for progesterone uptake by poly(dimethyldiallyl ammonium chloride) (PDDA )/poly (acrylic acid) (PAA) coated slides. Estradiol binding was greatest with poly(allylamine) hydrochloride (PAH)/PSS (0.03+/–0.01 to 1.54 pg/slide) and PDDA/ poly(styrenesulfonate) (PSS) (0.03+/–1 to 1.3+/–0.07 pg/slide) slides prepared in the presence of salt. For progesterone, binding was greatest with PAH/PSS (4+/–0.2 to 97+/–12 pg/slide), PAH/PAA (5+/–1 to 64+/–10 pg/slide) and PDDA/PSS (5+/–0.8 to 74+/–7 pg/slide) slides prepared in the presence of salt. Lutenizing hormone was taken up by the lenses and uptake was increased 2–3 fold in PAA coated lenses. Conclusions: Uptake of steroid and peptide hormones is possible with polyelectrolyte coated surfaces. Coated lenses showed enhanced hormone uptake. Contact lenses with specialized coatings may be a viable, noninvasive, in vivo option for monitoring hormone levels.

Keywords: contact lens • anterior segment • cornea: basic science 
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