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P. Segu, B. Hughes, S. Narayanan, F.P. Carney, C. Morris, M. Venugopal, L. Chapoy, Y. Lvov, A. McDermott, J.P. Bergmanson; Contact Lenses as a Diagnostic Tool II: Hormone Uptake by Poly–Electrolyte Coated Surfaces and Contact Lenses . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2073.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Contact lenses may be useful collection devices for detecting various analytes in the tear film. With a view to determining their potential to assist in monitoring fertility, we have investigated if hormones are taken up by poly electrolyte coated slides and lenses. Methods: Glass slides were coated with different charged coatings prepared in the presence or absence of salt using layer–by–layer technology. The slides were incubated with either 3H–estradiol (2–3000pg/ml) or 3H–progesterone (5–100nM) diluted in phosphate buffered saline or artificial tear solution for 2 hours at room temperature with shaking. The slides were stored over night at 4oC, then the amount of hormone bound was determined by liquid scintillation counting. Nelfilcon A lenses with or without coatings were soaked in lutenizing hormone (0.1ug/ml – 0.5mg/ml) overnight, washed, and the amount of hormone bound was detected by enzyme immunoassay. Results: All coated slides showed higher hormone binding than control uncoated slides except for progesterone uptake by poly(dimethyldiallyl ammonium chloride) (PDDA )/poly (acrylic acid) (PAA) coated slides. Estradiol binding was greatest with poly(allylamine) hydrochloride (PAH)/PSS (0.03+/–0.01 to 1.54 pg/slide) and PDDA/ poly(styrenesulfonate) (PSS) (0.03+/–1 to 1.3+/–0.07 pg/slide) slides prepared in the presence of salt. For progesterone, binding was greatest with PAH/PSS (4+/–0.2 to 97+/–12 pg/slide), PAH/PAA (5+/–1 to 64+/–10 pg/slide) and PDDA/PSS (5+/–0.8 to 74+/–7 pg/slide) slides prepared in the presence of salt. Lutenizing hormone was taken up by the lenses and uptake was increased 2–3 fold in PAA coated lenses. Conclusions: Uptake of steroid and peptide hormones is possible with polyelectrolyte coated surfaces. Coated lenses showed enhanced hormone uptake. Contact lenses with specialized coatings may be a viable, noninvasive, in vivo option for monitoring hormone levels.
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