May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
The Effect of MB500 on Corneal Epithelium and Keratocyte as Hyaluronan Biosynthesis Promoter: in vitro and in vivo Study
K.C. Shin; Y.S. In; J.–H. Ko; M.–G. Park; H.–G. Kim; W.R. Wee; J.H. Lee; M.K. Kim
Author Affiliations & Notes
  • K.C. Shin
    Department of Ophthalmology, Seoul National University collage of medicine, Seoul, Republic of Korea
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Y.S. In
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • J.–H. Ko
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • M.–G. Park
    MD Bioalpha, Daejeon, Republic of Korea
  • H.–G. Kim
    MD Bioalpha, Daejeon, Republic of Korea
  • W.R. Wee
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • J.H. Lee
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • M.K. Kim
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  K.C. Shin, None; Y.S. In, None; J. Ko, None; M. Park, MD bioalpha E; H. Kim, MD bioalpha E; W.R. Wee, None; J.H. Lee, None; M.K. Kim, None.
  • Footnotes
    Support  SNUH Grant 04–2004–019
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 2135. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      The Effect of MB500 on Corneal Epithelium and Keratocyte as Hyaluronan Biosynthesis Promoter: in vitro and in vivo Study
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      K.C. Shin, Y.S. In, J.–H. Ko, M.–G. Park, H.–G. Kim, W.R. Wee, J.H. Lee, M.K. Kim; The Effect of MB500 on Corneal Epithelium and Keratocyte as Hyaluronan Biosynthesis Promoter: in vitro and in vivo Study . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2135.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: To investigate the effect of MB500(MDbioalpha, Daejeon, Korea) on cultured human corneal epithelial cells and keratocytes and to evaluate the effect of MB500 on corneal wound healing in rabbit. Methods: Various concentrations(1–200uM) of MB 500(10ul) was applied to the cultured human epithelial cells and keratocytes. Supernatant was collected after 48 hours of incubation with MB 500. MTT assay was performed to evaluate toxicity of MB 500 to the cells. Hyaluronan(HA) synthesis assay with biotinylated hyaluronan binding protein(b–HABP), glycosaminoglycan(GAG) synthesis assay and nitric oxide(NO) assay were performed using ELISA. Epithelial defects were made using 7.0 mm trephine and application of 20% alcohol for 20 seconds in 24 rabbits. MB500(200uM) or phosphate buffer saline(PBS) as a control was topically instilled four times a day to each 12 rabbit’s eye. The ratio of the epithelial defect size to the total corneal area was calculated using Image Pro PlusTM(Media Cybernetics, MD, USA) every day until the wound was completely healed. Hyaluronan was stained with b–HABP and 3,3’–diaminobenzidine dihydrochloride at 1,2 and 5 days after injury in both treated group and control Results: Cell death was not increased in MB500–treated cells(1uM and 200uM) compared with that in control. HA increased by 1.15 folds in epithelial cell with 5uM of MB500 and by 1.35 folds in keratocytes with 5uM of MB 500. 50uM of MB500 increased the concentration of GAG by 2 folds. MB500 decrease NO concentration by 43% at 50uM compared with that in positive control with IL–1ß. Corneal wound healing is rapid in study group with MB500 compared with control group with PBS and statistically significant difference(p=0.034) is observed at 3 days after injury. Conclusions: The instillation of MB500 shows no cell toxicity. MB 500 can promote corneal wound healing possibly by increasing HA and GAG and by decreasing NO.

Keywords: cornea: basic science • cornea: epithelium • cornea: stroma and keratocytes 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept