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K. Terai, Y. Hayashi, T.–I. Chikama, N. Terai, W.W. Kao; Role of TGF–ß Signaling in Wound Healing of Corneal Epithelium . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2144.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To examine the roles of TGF–ß signaling pathways in regulating cell migration and proliferation of the healing of corneal epithelium debridement. Methods: TGF–ß type II receptor (TbR2) floxed mice were bred with Krt12–Cre mice to generate bitransgenic mice in which the TGF–ß receptor 2 gene was disrupted selectively in the corneal epithelial cells. Corneal epithelial debridement (2 mm diameter) was created in 2–month–old bitransgenic Krt12Cre/CreTbR2f/f mice and their littermates as controls Krt12Cre/CreTbR2f/+ and Krt12Cre/CreTbR2+/+. The healing of corneal epithelium defect was assessed by fluorescein staining at different intervals of debridement. The experimental mice were administrated by i.p. injection of BrdU two hours prior to sacrifice to determine cell proliferation. Immunohistochemistry was performed to elucidate potential signal transduction molecules involved. Results: The corneal epithelium of Krt12Cre/CreTbR2f/f mice lost TbR2 as judged by western blot analysis with anti–TbR2 antibodies and exhibited delayed healing of debridemnt, in comparison to control littermates that were heterozygous floxed and wild typeTbR2. The naïve uninjured corneal epithelium of Krt12Cre/CreTbR2f/f mice exhibited higher cell proliferative activities than controls. Corneal epithelium debridement caused cessation of epithelial cell proliferation in all three groups of experimental mice in 6–12 h. Immunohistochemistry using anti–phospho–p38 revealed a significant decrease of phosphorylation of p38 in 6–12 h of debridement. Conclusions: Our results indicate that the TGF–ß signals play a critical role in the corneal epithelial wound healing, especially in the migration of corneal epithelial cells.
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