Purchase this article with an account.
T. Blanco–Mezquita, J. Merayo–LLoves, 1B, R. Torres, H. Martinez–Osorio, 1A, S. Bonini, S. Mar–Sardaña, A. Lambiase; NGF Treatment and Corneal Wound Healing Process . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2145.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: The purpose of this research is to evaluate the effectiveness of topical administration of nerve growth factor in the wound healing process after refractive surgery and to prove that NGF–TrkA interaction decrease earlier apoptosis inhibiting mitochondrial cytochrome c release, in both culture and in vivo conditions. Methods: Iber Brown hens underwent PRK were divided into different groups treated with topical administration of 0.2% murine NGF group A, balanced salt solution (BSS) group B and group C received no treatment. Clinical monitoring and transparence measurements were taken. Eyes were exenterated at sequential time points. Sections were stained with H–E. TUNEL technique was used for apoptosis detection. TrkA receptors and cytochrome c were detected by immunohistochemistry and westerm blot assay. Culture keratocytes was performed in presence of 0,1 µg/ml of Murine NGF (NGF group) and absence of NGF (CONTROL group). Proliferation in culture keratocytes was mean by incorporation of 3H–timidine and keratocyte apoptosis was evaluated by flow cytometry with annexin V FITC Results: The appearance and disappearance of the Haze process was more quickly in time in group A than both B and C groups. TrkA receptor was found in epithelium, endothelium and keratocytes in all the corneas. In the first hours after surgery, apoptosis and mitochondrial cytocrhrome c release were lesser in group A than both B and C groups. Proliferation in culture keratocytes was higher in NGF group than CONTROL group. Apoptosis and mitochondrial cytocrhrome c release were lesser in NGF group than CONTROL group. Conclusions: TrkA receptors are present in the cornea of hens NGF inhibits mitochondrial cytocrome c release, in both culture and in vivo assays which probably causes a decreases of keratocyte apoptosis. NGF stimulates proliferation in culture cells NGF accelerates the wound healing procces. The hen is a very good model to study corneal wound healing after refractive surgery
This PDF is available to Subscribers Only