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T.D. Washington, K.S. Burney; Transport of Poly (lactic–co–glycolic acid) Particles Containing Antisense Oligonucleotides by Iontophoresis: An in vitro Study . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2164.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Research in corneal drug delivery has shown transport across the corneal tissue layers to be a barrier for effective genetic therapeutic treatment. Transport of the CTGF antisense oligonucleotides to treat corneal scarring can benefit from using polymeric carriers to extend bioavailability and protect from enzymatic degradation. The rate of particle diffusion across the cornea is further enhanced by the addition of iontophoresis. The current study was designed to investigate the permeability of the cornea to antisense oligonucleotides within carriers and to demonstrate an increase in transport efficiency of the oligonucleotides with iontophoresis. Methods: Eight rabbit corneas were mounted between two horizontal diffusion cells. Half were exposed to a 1mM solution of fluorescently labeled oligonucleotides and half were exposed to a 0.60 % solution of PLGA particles at a constant temperature of 35°C. Two corneas from each group were subjected to iontophoresis at 1.5mA for 10 minutes. A fluorescent plate reader was used to determine the concentration of particles present. The confocal microscope was used to confirm the presence of oligonucleotides and microspheres. Results: There is a higher concentration of oligonucleotides present in the receiver solution after iontophoresis. Conclusions: The results suggest that iontophoresis increases the rate of particle transport across the cornea.
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