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T. Miyamoto, S. Saika, Y. Okada, K. Fujita, Y. Ohnishi; NF Kappa B Inhibitor Prevents the Inflammatory Cell Migration Through the Inhibition of MIP1a Expression in Corneal Penetrating Injury in Rats . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2642.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the therapeutic efficacy of SN50, nuclear factor kappa B inhibitor, in corneal penetrating injury model in rats. Methods: The animal protocol was approved by National Cancer Institute / NIH. Adult rats (n=28) were generally and topically anesthetized and the cornea of the right eye was pierced by a hypodermic needle with/without injection of SN50 to the anterior chamber. After intervals of healing from 2 hr up to 3days the animals were killed. The eyes were processed for hematoxylin– eosin staining and immunohistochemistry for macrophage chemoattractant protein 1 (MCP1) and macrophage inflammatory protein 1alpha (MIP1a). Results:In control group, marked migration of neutrophils was detected in anterior chamber from 6 to 12hours after corneal penetrating injury, and macrophages were detected in corneal stroma at 24hour. In SN50 group, both of neutrophils migration and macrophages invasion were less detected compared with control group. In immunohistochemistry, MIP1a expression in the corneal epithelium was detected in control group, but not in SN50 group. MCP1 expression in the corneal epithelium was detected in both of control and SN50 group. Conclusions:NFkB inhibition after corneal penetrating injury suppresses the expression of MIP1a in corneal epithelium, and prevents the migration of inflammatory cells in corneal stroma and anterior chamber.
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