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K.L. Moeller, C.C. Chan, J. Hackett, M.A. Crawford, S. Morrison, J.A. Smith; Conjunctival Epithelial Keratinization Is a Pathologic Feature of Ocular Surface Disease in Premature Ovarian Failure . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2682.
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© ARVO (1962-2015); The Authors (2016-present)
To describe conjunctival epithelial keratinization in 2 types of ocular surface disease and correlate to symptoms of ocular surface irritation.
123 women: 18 normal controls (NV), 62 with Premature Ovarian Failure (POF), and 43 with Sjogren’s Syndrome (SS) underwent standardized slit lamp exam and dry eye tests including Schirmer and tear breakup time. Symptoms were assessed with the ocular surface disease index questionnaire, (OSDI) ©. Conjunctival epithelial cells were obtained using Dacron swabs and processed for cytology via cytospin centrifugation. Cytology specimens were stained with Giemsa or fixed and processed for transmission electron microscopy (TEM), with JEOL JEM–1010. Immunohistochemistry was performed to detect CK10, a marker of keratinization using avidin–biotin–complex immunostaining method. The Kruskal–Wallis test was used to compare the median proportions of positively stained cells between the disease categories.
Conjunctival epithelial keratinization is a pathologic feature of ocular surface diseases such as SS and we now demonstrate this abnormality in women with POF. Keratinization can cause symptoms of irritation in ocular surface disease. Anti– keratinization therapies may be useful to improve ocular surface health and decrease irritation in patients with POF.
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