May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
A New Donor Cornea Harvesting Technique : for Posterior Lamellar Keratoplasty
O. Suwan–apichon; M. Rizen; J.M. G. Reyes; S. Herretes; A. Behrens; W.J. Stark; R.S. Chuck
Author Affiliations & Notes
  • O. Suwan–apichon
    Wilmer Ophthalmological Institue, Johns Hopkins University, Baltimore, MD
    Deparment of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • M. Rizen
    Wilmer Ophthalmological Institue, Johns Hopkins University, Baltimore, MD
  • J.M. G. Reyes
    Wilmer Ophthalmological Institue, Johns Hopkins University, Baltimore, MD
  • S. Herretes
    Wilmer Ophthalmological Institue, Johns Hopkins University, Baltimore, MD
  • A. Behrens
    Wilmer Ophthalmological Institue, Johns Hopkins University, Baltimore, MD
  • W.J. Stark
    Wilmer Ophthalmological Institue, Johns Hopkins University, Baltimore, MD
  • R.S. Chuck
    Wilmer Ophthalmological Institue, Johns Hopkins University, Baltimore, MD
  • Footnotes
    Commercial Relationships  O. Suwan–apichon, None; M. Rizen, None; J.M.G. Reyes, None; S. Herretes, None; A. Behrens, None; W.J. Stark, None; R.S. Chuck, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 2693. doi:
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      O. Suwan–apichon, M. Rizen, J.M. G. Reyes, S. Herretes, A. Behrens, W.J. Stark, R.S. Chuck; A New Donor Cornea Harvesting Technique : for Posterior Lamellar Keratoplasty . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2693.

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Abstract

Abstract: : Purpose: To describe a technique for posterior lamellar keratoplasty donor preparation. Methods: Eight human donor research corneas were mounted onto an artificial anterior chamber and deep stromal pockets dissected. Four corneas were mounted in the standard endothelial side down orientation and dissected using standard instruments [Group 1]. Another four corneas were mounted endothelial side up and dissected using a flat spatula [Group 2]. Trephined lamellar graft thickness was assessed by ultrasound pachymetry. The grafts were also analyzed using vital staining of the endothelium and standard histological preparation. Results: Achieved posterior graft thickness was 118+32 microns [Group 1] and 92+23 microns [Group 2] (p=0.324). Percentage of devitalized endothelial cells was 0.86+1.48 % [Group 1] and 3.9+2.9 % [Group 2] (p=0.185). The dissections using both harvesting techniques remained in plane and were smooth. Conclusions: A blunt spatula and endothelium side up orientation on an artificial anterior chamber can be used to create posterior lamellar dissections without compromising endothelial cell number or planarity when compared to standard endothelium side down harvest.

Keywords: cornea: endothelium • cornea: clinical science • cornea: endothelium 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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