Abstract:
: To establish accuracy and reproducibility of theINTALASE Femtosecond laser in performing lamellar corneal sectionswith special attention to thickness and diameter of the button
Twenty eight human donor corneas underwent lamellarcorneal sections. Intended thicknesses were 225um to 500um.Intended diameters were 6.0mm to 9.0mm. The incision patternswere spiral and Raster. Energy was 3.2mJ: the side cut anglewas 90°. The corneas were mounted on the Moria artificialanterior chamber without suction ring. Thickness was measuredwith Vernier calipers. Diameter was measured off digital imagesusing the Scioncorp software. Two laser systems were used toassess reproducibility between them. Surface quality was assessedwith SEM
Table 1. Achieved corneal discs thickness (um).
Table 2. Achieved corneal discs diameters (mm).
The raster pattern provided a better surface quality as demonstratedby scanning electron microscopy
The accuracy, reproducibility, and surface smoothness are acceptablefor clinical use. Further refinements of the technique willreduce ovalization of the button shape.
Keywords: laser • transplantation • microscopy: electron microscopy