May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Modified Microkeratome–Assisted Posterior Lamellar Keratoplasty Using a Tissue Adhesive as Sealant
A. Pirouzmanesh; S. Herretes; J.M. G. Reyes; O. Suwan–Apichon; R.S. Chuck; D.–A. Wang; J.H. Elisseeff; W.J. Stark; A. Behrens
Author Affiliations & Notes
  • A. Pirouzmanesh
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • S. Herretes
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • J.M. G. Reyes
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • O. Suwan–Apichon
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • R.S. Chuck
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • D.–A. Wang
    Departments of Cell Biology and Biomedical Engineering, Johns Hopkins University, Baltimore, MD
  • J.H. Elisseeff
    Departments of Cell Biology and Biomedical Engineering, Johns Hopkins University, Baltimore, MD
  • W.J. Stark
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • A. Behrens
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • Footnotes
    Commercial Relationships  A. Pirouzmanesh, None; S. Herretes, None; J.M.G. Reyes, None; O. Suwan–Apichon, None; R.S. Chuck, None; D. Wang, None; J.H. Elisseeff, None; W.J. Stark, None; A. Behrens, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 2713. doi:
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      A. Pirouzmanesh, S. Herretes, J.M. G. Reyes, O. Suwan–Apichon, R.S. Chuck, D.–A. Wang, J.H. Elisseeff, W.J. Stark, A. Behrens; Modified Microkeratome–Assisted Posterior Lamellar Keratoplasty Using a Tissue Adhesive as Sealant . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2713.

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Abstract

Abstract: : Purpose: To compare graft stability and astigmatic change using suture vs. tissue adhesive in an experimental model of microkeratome–assisted posterior lamellar keratoplasty (PLK). Methods: A 300–µm thick partial flap–keratectomy was performed in human donor corneoscleral rims using an artificial anterior chamber and a manual microkeratome. The flap stopped at the left central opening border, providing a wide hinge to add stability. After flap reflection, a 6.25 mm trephination was performed obtaining a disc of posterior stroma, Descemet’s membrane, and endothelium. The disc was positioned in a sutureless fashion, and the flap secured with either 5 interrupted sutures, or a chondroitin–sulfate–aldehyde based adhesive. Increasing intrachamber pressures were created detecting graft stability. Videokeratographic data was recorded evaluating astigmatic change. Results: The mean astigmatic change was 3.08 D (0.84) in the sutured group, and 1.13 D (0.55) in the glued group (p=0.008). Mean resisted pressures were 95.68 (27.38) mmHg and 82.45 (18.40) mmHg in the sutured and glued groups, respectively (p=0.97). Conclusions: This modified technique of microkeratome–assisted PLK showed excellent graft stability in both groups. Flaps sealed with the novel tissue adhesive had reduced astigmatic changes in our experimental model.

Keywords: cornea: endothelium • cornea: stroma and keratocytes 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
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