Abstract
Abstract: :
Purpose: To analyze, in vitro, the antimicrobial properties of the polymerization process of the Ethyl–cyanoacrilate (Superbonder®, Loctite) and the n–Butyl–cyanoacrylate (Hystoacryl®, B. Braun) in different microorganisms related to corneal infections. Methods: We analyzed the following microorganisms: (1) Staphylococcus aureus (ATCC 25923) (10 plates); (2) Streptococcus pneumoniae (20 plates); (3) Pseudomonas aeruginosa (ATCC 27853) (10 plates); and (4) Escherichia coli (ATCC25922) (10 plates). The same volume of n–Butyl–cyanoacrylate and Ethyl–cyanoacrylate were dropped directly to a blank disk. Each plate had one sample of (1) n–Butyl–cyanoacrylate polymerized outside the plate; (2) n–Butyl–cyanoacrylate polymerized on the plate (3) Ethyl–cyanoacrylate polymerized outside the plate; (4) Ethyl–cyanoacrylate polymerized on the plate; and a (5) control disk. The plates were incubated at 35 ± 2°C and its growth examined after 24 hours. To determine the bactericidal activity of the glue, a sample inoculum was taken from within the zone of inhibition and was re–cultured. These plates were examined after 24 and 48 hours. All samples were duplicated. Results: For Staphylococcus aureus, all dishes showed growth inhibition and the n–Butyl–cyanoacrylate polymerized inside the plate has a significant inhibition (p<0.0001) compared to the n–Butyl–cyanoacrylate polymerized outside the plate. For Streptococcus pneumoniae, all dishes showed growth inhibition and both cyanoacrylates polymerized inside the plate have a significant inhibition compared to the cyanoacrylates polymerized outside the plate (p<0.05). For the Escherichia coli, only the Ethyl–cyanocrylate sample polymerized inside the plate had growth inhibition (11.2± 2.66mm). No growth inhibition was observed in the plates with Pseudomonas aeruginosa. Regarding the bactericidal activity of the Staphylococcus aureus, Streptococcus pneumoniae and Escherichia coli is respectively 10%, 60.25% and 30%. Conclusions: The polymerization process of the cyanoacrylates showed, in vitro, to have an important role in the antimicrobial effect against Staphylococcus aureus, Streptococcus pneumoniae and Escherichia coli.
Keywords: microbial pathogenesis: experimental studies • Staphylococcus