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Y. Jin, L. Shen, L. Chen, Q. Zhang, R. Dana; Expression of CCR7 by Corneal Antigen–Presenting Cells in Inflammation . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2810.
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Purpose: CCR7 and its ligands, the chemokines CCL19 (MIP–3beta) and CCL21 (SLC), have been related specifically to mobilization and emigration of mature MHC class II+ antigen–presenting cells (APCs) from sites of inflammation to draining lymph nodes (LN) for priming of naïve T cells. Experiments were undertaken to examine whether CCR7 and its ligands are involved in the mobilization of corneal APCs in the inflamed corneal microenvironment. Methods: We placed intrastromal corneal sutures to induce corneal inflammation in BALB/c mice. To determine whether APCs express CCR7 in the inflamed cornea, immunohistochemical staining for CCR7 and its ligands CCL21, CCL19, along with surface markers CD45, CD11b and Iad was done and observed by confocal microscopy on whole–mounted corneas. Additionally, corneal buttons were placed in culture ex vivo to harvest adherent and non–adherent cell populations to examine for surface expression of CCR7 by flow cytometry. Results: CCR7+ cells were found in the inflamed neovascularized corneas one week after suture placement. All of these cells were located around the lymphatic vessels growing into the corneal periphery. Double–staining showed these cells were 100% CD11b+ and MHC class II (Iad )+. Additionally, expression of CCR7 was present on APCs harvested from cornea buttons ex vivo. Conclusions: These data demonstrate that CCR7 is expressed on the surface of mature corneal APCs in the inflamed corneal microenvironment. This finding suggests that CCR7 and its ligands may play a role in the mobilization of corneal APC into secondary lymphoid tissues in inflammation.
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