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D. Kurosaka, M. Yoshino, K. Nakamura, K. Negishi, K. Tsubota; Adhesive Property of an Intraocular Lens Influences Lens Epithelial Cell Migration Under the Optic . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2853.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To determine whether adhesive properties of intraocular lenses (IOLs) influenced migration of lens epithelial cells (LECs) under the optic. Methods: Porcine LECs were cultured in an insert, containing a collagen membrane, with various IOL optics (three types of acrylic, two of silicone, and one of PMMA). In this culture system, only the center of the optic was attached to the membrane. To prevent early LEC migration onto the collagen membrane under IOL optic, the IOL optic was pressed tightly to the membrane by a weight. The weight was removed 48 hr after culture initiation; then culture was continued. The cell–free area into which LECs initially could not migrate was measured after removing the weight and again after 48 hr. A migration ratio – the area where LECs were newly present at 48 hr divided by the cell–free area immediately after weight removal – was caluculated. Adhesion between the collagen membrane and the IOL optic was measured directly with a tensiometer. Results: When the IOL has pressed to the collagen membrane with a 510–mg weight, LEC migration into the space between the optic and the membrane was inhibited. After weight removal, LECs migrated to various extents into the space (p=0.0001, ANOVA). Migration ratios ranged from 3.1 ± 3.2% for MA60BM (acrylic) to 17.1 ± 4.0% for NPD74D (PMMA). A significant difference in maximum tensions was evident between IOL optics (p=0.0060; ANOVA). The migration ratio was negatively related to maximum tension (p=0.0476; Spearman rank correlation coefficient). Conclusions: LEC migration under the IOL optic was related to the adhesive property of the IOL optic.
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