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K. Tanemoto, Y. Goto, N. Ibaraki, T. Miyamoto, A. Akatsuka; Expression of Glucocorticoid Receptor in Human Lens Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2872.
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Purpose: Although it is well known that long–term steroid administration can cause cataract, there is controversy concerning the mechanism that leads to steroid–induced cataract. It has been hypothesized that the cataract is caused either by the direct action of the steroid on the lens or is due to a secondary effect related to the action of the steroid on other organs. Previous studies using biochemical techniques have shown that bovine and chicken lenses do not recognize the glucocorticoid receptor (GR). Thus it is thought that other organs, which do have GRs, are responsible for the production of lipid peroxide and the accompanying oxidized stress that causes cataract. The purpose of this study is to examine the expression of GR in human lens epithelial cells (HLECs) through the use of RT–PCR and immunohistochemistry. Methods: HLECs with anterior capsules were obtained during cataract extractions. The RNA from the HLECs was either immediately extracted or extracted after the HLECs were cultured in DMEM for 3 weeks. RT–PCR was performed using a TITANIUM one–step RT–PCR kit. Human whole lenses extracted from lens dislocated patients and posterior capsule opacification (PCO) specimens were fixed with formalin. Paraffin sections were created and these sections were immunostained with anti–GR antibody. The HLECs that were cultured for 3 weeks were fixed with methanol and immunostained with anti–GR antibody. Results: There was no GR positive staining in normal HLECs in vivo. In contrast, GR positive cells were found in the human PCO specimens and cultured HLECs. RT–PCR bands for GR were detected in both the normal HLECs in vivo and in the cultured HLECs. However, the bands for the normal HLECs were weaker than those seen for the cultured HLECs. Conclusions: The results suggest that surrounding conditions may influence the extent of the GR expression in HLECs.
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