May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Presence of 3–Oxidopyridinium Derivatives of Lysine in Human Lens
Author Affiliations & Notes
  • O.K. Argirov
    Mason Eye Institute, University Missouri–Columbia, Columbia, MO
  • B.J. Ortwerth
    Mason Eye Institute, University Missouri–Columbia, Columbia, MO
  • Footnotes
    Commercial Relationships  O.K. Argirov, None; B.J. Ortwerth, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 2886. doi:
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    • Get Citation

      O.K. Argirov, B.J. Ortwerth; Presence of 3–Oxidopyridinium Derivatives of Lysine in Human Lens . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2886.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Abstract:
 

 

In a previous study we reported that a compound having the structure 2 could be detected in digested human lenses using LS–MS/MS. We reported that the structure 2 could be formed by the combined action of glycolaldehyde and glyceraldehyde. Recently we found that structures 1 and 3 could be formed by the individual action of glycolaldehyde or glyceraldehyde on Boc–Lys in vitro. The purpose of this study was to find if structures 1 and 3 could be detected in digested human eye lenses.

 

 

Human lenses were subject of digestion using a set of proteolytic enzymes. 13C labeled standards of compounds 1 and 3 were added to the digest obtained. HPLC fractions containing the standards were collected. MS and MS/MS spectra of the fractions corresponding to the standards were recorded.

 

 

The MS and MS/MS spectra suggest that both the 13C labeled standards and the corresponding non–labeled products were present in the fractions collected. This is an evidence that structures 1 and 3 exist in human lens.

 

 

The presence of structures 1, 2 and 3 in lens proteins suggests that glycolaldehyde and glyceraldehyde are effective glycation agents in human eye lens according to the scheme given bellow.

 

 

 
Keywords: aging • cataract • protein modifications-post translational 
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