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K. Hirayama, Y. Hata, M. Miura, T. Kita, Y. Noda, T. Ishibashi; Syntheses and Regulation of Hyarluronan in Hyalocytes . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2965.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: The hyaluronan (HA) is one of the major components of vitreous under physiological condition, and is also suggested to plays a role as a scaffolding of migrating cells at a site of tissue remodeling. We investigated the synthesis and expressional regulation of HA by hyalocytes. Methods: The hyalocytes and capillary endothelial cells (EC) were isolated from bovine vitreous and retina, respectively. The cells were stimulated with platelet–derived growth factor (PDGF) –BB or transforming growth factor (TGF)–beta, both of which are considered to be involved in the pathogenesis of vitreo–retinal interface diseases. The conditioned media were subjected to enzyme–liked immunosorbent assay (ELIZA) to examine the secretion of HA. The expression of hyaluronan synthase (HAS) 1–3 gene was also analyzed by reverse transcriptional polymerase chain reaction (RT–PCR). Results:Both EC and hyalocytes were capable of secreting HA into the conditioned media. While the secretion of HA by EC was not affected by cytokines examined, its secretion by hyalocytes was significantly enhanced by PDGF–BB and TGF–beta. The hyalocytes expressed HAS1–3 gene and the expression of these gene was strengthened in the presence of TGF–beta. Conclusions: The hyalocytes seem to be one of the cells which synthesize HA in the vitreous cavity under physiological condition. It is also suggested that the hyalocytes might be involved in the pathogenesis of proliferative vitreoretinal diseases through the production of HA.
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