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S.A. Keirstead, J.M. Reifel Salzberg; Puringergic Responses in Acutely Isolated Rat Muller Cells From Uninjured and Optic Nerve Transected Retinas . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2968.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To determine if purinergic receptors are up–regulated in rat Muller cells after optic nerve transection. Methods: Under surgical anesthesia one optic nerve of Long Evans pigmented rats was completely transected intraorbitally. Four days to 95 weeks after optic nerve transection retinas were removed and enzymatically and mechanically dissociated. Isolated Muller cells were loaded with Fura–2 and calcium imaging was performed using a fluorescence microscope equipped with a CCD and computer controlled acquisition and analysis system. Results: Muller cells responded in a dose–dependent manner to 1–100 uM of the purinergic receptor agonists ATP, 2MeSATP, 2MeSADP, and ADP, but not the pyrimidines UTP or UDP. Purinergic agonists evoked an increase in intracellular calcium in the endfoot and proximal shaft, and rarely in the soma or distal shaft. Muller cells isolated from retinas after optic nerve transection responded similarly to purinergic agonists. There was no evidence that a larger proportion of Muller cells from the injured retinas responded to purinergic agonists than did those from intact retinas. Conclusions: Muller cells do not up–regulate purinergic receptors after optic nerve transection.
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