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J. Tong, Y. Shen, D.S. C. Lam, C.P. Pang; Effects of Triamcinolone Acetonide on VEGF and PEDF Expressions in Human Retinal Pigment Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3031.
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Purpose: Individual, multiple, and interactive effects of growth factors, including vascular endothelial growth factor (VEGF) and pigment epithelial derived factor (PEDF), affect blood vessels growth inwards the choroids in animal eyes. In human choroidal neovascularization (CNV) refers to the formation of new blood vessels from the choriocapillaries through Bruch’s membrane into the subretinal space. Intravitreal triamcinolone (TA) stabilizes recurrent CNV in patients. We investigated whether TA affects VEGF and PEDF in cultured human retinal pigment epithelial (ARPE19) cells. Methods:We studied the time course of TA regulated VEGF and PEDF mRNA in cultured ARPE19 cells, which were grown to subconfluence and then treated with TA. VEGF and PEDF expressions were determined at time points from time 0 to 3 days by RT–PCR, real–time PCR and ELISA. Results: TA treatment led to significant reduction of the RPE cells. VEGF expression was decreased by about 50% from time 10 min but returned to levels of the controls after 24 hours. At the same time the expression of PEDF was increased by about 2.5 times, which also returned to levels of the control after 24 hours. Changes in the respective protein levels were consistent. Conclusions: TA cytotoxicity was confirmed in our cultured RPE cells. TA reduces the expression of VEGF, but induces the expression of PEDF. These observations may suggest an antagonistic mechanism of these two growth factors that account for the inhibition of CNV by TA.
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