May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Effects of Triamcinolone Acetonide on VEGF and PEDF Expressions in Human Retinal Pigment Epithelial Cells
Author Affiliations & Notes
  • J. Tong
    Zheyi Eye Center, The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, China
    Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  • Y. Shen
    Zheyi Eye Center, The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, China
  • D.S. C. Lam
    Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  • C.P. Pang
    Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  • Footnotes
    Commercial Relationships  J. Tong, None; Y. Shen, None; D.S.C. Lam, None; C.P. Pang, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 3031. doi:
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      J. Tong, Y. Shen, D.S. C. Lam, C.P. Pang; Effects of Triamcinolone Acetonide on VEGF and PEDF Expressions in Human Retinal Pigment Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3031.

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Abstract

Abstract: : Purpose: Individual, multiple, and interactive effects of growth factors, including vascular endothelial growth factor (VEGF) and pigment epithelial derived factor (PEDF), affect blood vessels growth inwards the choroids in animal eyes. In human choroidal neovascularization (CNV) refers to the formation of new blood vessels from the choriocapillaries through Bruch’s membrane into the subretinal space. Intravitreal triamcinolone (TA) stabilizes recurrent CNV in patients. We investigated whether TA affects VEGF and PEDF in cultured human retinal pigment epithelial (ARPE19) cells. Methods:We studied the time course of TA regulated VEGF and PEDF mRNA in cultured ARPE19 cells, which were grown to subconfluence and then treated with TA. VEGF and PEDF expressions were determined at time points from time 0 to 3 days by RT–PCR, real–time PCR and ELISA. Results: TA treatment led to significant reduction of the RPE cells. VEGF expression was decreased by about 50% from time 10 min but returned to levels of the controls after 24 hours. At the same time the expression of PEDF was increased by about 2.5 times, which also returned to levels of the control after 24 hours. Changes in the respective protein levels were consistent. Conclusions: TA cytotoxicity was confirmed in our cultured RPE cells. TA reduces the expression of VEGF, but induces the expression of PEDF. These observations may suggest an antagonistic mechanism of these two growth factors that account for the inhibition of CNV by TA.

Keywords: choroid: neovascularization • drug toxicity/drug effects 
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