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M. Suzuki, T. Kanamoto, S. Tanimoto, H. Tamura, H. Okumichi, H.K. Mishima; The Association of Wnt9/14 With Chick Retina Development . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3124.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the molecular and biological function of Wnt 9/14 in chick retina development. Methods: Proteomics analysis of chick embryo retina, embryonic day7(E7) and day11(E11), were performed, and the up–regurated proteins, including Wnt 9/14, were identified by mass–spectrometry. The change of Wnt 9/14 expressions were examined using Western blot analysis. Immunohistochemical procedure were performed to examine the localization of the proteins. Results: Wnt 9/14 was more highly expressed in matured stage(E11) than pre–differentiated stage(E7) of chick retina. The up–reguration of Wnt 9/14 expression was also confirmed by Western blot. Moreover, retinal ganglion cells have the high tendency of Wnt 9/14 specific expression in histologic and fluorescence microscopic examinations. Conclusions: This study suggest that Wnt 9/14 may have an important role of retinal differentiation.
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