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S. Zareparsi, H. Cheng, S. Shah, S. Yoshida, M. Othman, J. Xi, U.P. Andley, A. Swaroop; Gene Expression Profiles of Crystallins During Development and Aging in Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3141.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Crystallins constitute the major proteins of the vertebrate eye lens and are essential for maintaining the transparency and refractive index of the lens. However, very little is known about the function of crystallins in the retina, where their expression has recently been reported by several studies. The goal of this study is to conduct a comprehensive survey of the expression pattern of the members of the crystallin family during development and aging in mice retina. Methods: Retina was isolated from C57BL/6 mice at different stages during development (embryonic, postnatal, adult) and aging (2,16, 21 months). Gene–specific primers were utilized for quantitative RT–PCR. In–situ hybridization was performed to verify the differential expression of two of the crystallins during aging in retina. Immunoblot analysis was carried out with retina isolated from postnatal (P0, P21) and 10 month old 129Sv mice. Immunolocalization studies were performed to define the expression of crystallins in different retinal layers. Results: Several members of the crystallin family are differentially expressed in mice retina during both development and aging. During development, a novel αA–crystallin exhibited a unique pattern of gradual increased expression starting at P0, with the highest level observed at P21. During aging, many different crystallins were down–regulated dramatically with the highest levels of decrease seen in αA–crystallin and γD–crystallin. In–situ hybridization confirmed the reduced expression of ßA1–crystallin and γS–crystallin during aging in retina. Conclusions: Our results suggest that crystallins play an important role in retina during both differentiation and aging. During development, they may be involved in cellular signaling pathways and regulation of other genes involved in photoreceptor development. While in aging, the down–regulation may be associated with reduced function and accumulation of protein aggregates.
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