May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Expressin of Clusterin on Developing Retina: Association With Retinal Vessel Development
Author Affiliations & Notes
  • Y.S. Yu
    Ophthalmology, Seoul Natl Univ Coll of Med, Seoul, Republic of Korea
  • J. Kim
    Ophthalmology, Seoul Natl Univ Coll of Med, Seoul, Republic of Korea
  • S. Jeong
    Ophthalmology, Seoul Natl Univ Coll of Med, Seoul, Republic of Korea
  • J. Kim
    Biochemistry, Seoul Natl Univ Coll of Pharmacy, Seoul, Republic of Korea
  • Y. Choi
    Biochemistry, Seoul Natl Univ Coll of Pharmacy, Seoul, Republic of Korea
  • K.–W. Kim
    Biochemistry, Seoul Natl Univ Coll of Pharmacy, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Y.S. Yu, None; J. Kim, None; S. Jeong, None; J. Kim, None; Y. Choi, None; K. Kim, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 3163. doi:
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      Y.S. Yu, J. Kim, S. Jeong, J. Kim, Y. Choi, K.–W. Kim; Expressin of Clusterin on Developing Retina: Association With Retinal Vessel Development . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3163.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To elucidate the association of clusterin expression with retinal vessel development Methods: At P0, 2, 4, 6, 8, 10, 12, 14, 16, 18, and 26 of C57BL/6 mice, immunohistochemistry and western blotting for clusterin, Hsp70, PECAM, ZO–1, Flt–1, Flk–1, RACK–1, and TUNEL were performed in the retina Results: PECAM, ZO–1, Flt–1, Flk–1 was expressed in the inner retina and increased with the progression of vasculogenesis and angiogenesis. RACK–1 was decreased with increasing time. TUNEL was positive broadly in retina, and changed with increasing time. Hsp70, which was expressed primarily in outer retina, was weakly expressed till P8 when eyelids open, but increased after P10. Clusterin was prominently expressed in inner retina, and constantly up–regulated during vasculogenesis and angiogenesis Conclusions: Clusterin was prominently expressed in inner layer of developing retina, where active vascular development occurs. Clusterin expression was closely associated with retinal vascular formation. It may be a cellular chaperone for retinal vessel development.

Keywords: retinal development • vascular cells • chaperones 
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