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D.D. Sta Iglesia, J.C. Hammond, D.F. Valenta, M.E. Pease, H. Lai, H.A. Quigley; Incorporation and Survival of Ciliary Body–Derived Progenitor Cells From Adult GFP Mouse Into Rat Eyes With Glaucoma and With Transected Optic Nerves . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3220.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To assess variables that affect incorporation of ciliary body (CB)–derived progenitor cells into rat retina, including degree and type of injury to retinal ganglion cells, and time after transplantation. Methods: CB progenitor cells from adult mice transgenic for green fluorescent protein (GFP) were injected intravitreally into Wistar rat eyes (20,000 cells in 5 µl). Rats had experimental glaucoma (laser treatment model) for 1 or 3 months prior to transplant and 4 or 12 weeks survival after transplant (7–9 rats per group). Additional rats had optic nerve transection 1 or 3 months prior to transplant with 1–month survival. A masked observer counted transplanted cells in hemi–retinal flat mounts and cross–sections. Results: GFP fluorescent cells incorporation was similar 1 month and 3 months after transplant in glaucoma retinas (53 ± 40 vs 75 ± 25 cells/mm2, p = 0.133, t test), representing about 10% incorporation. Incorporation was similar with 1 or 3 months of glaucoma damage before transplantation (88 +/– 22 vs 147 +/– 70 cells/mm2, p = 0.1). Incorporation was not different in transplants 1 compared to 3 months after transection (41 ± 29 vs 58 ± 32, p = 0.40). Glaucoma eyes had more surviving GFP cells than transection eyes (p < 0.01). Incorporation was not related to severity of ganglion cell loss in glaucoma eyes, judged by number of remaining optic nerve axons (R2 = 0.33 , p = 0.097). Incorporation in the ganglion cell and nerve fiber layer was 5–10 times greater than in other retinal layers, unaffected by time since transplant (1 vs 3 months). Conclusions: Adult mouse CB progenitor cells survive for 3 months after transplant into rat retinas with experimental glaucoma. The number of fluorescent cells was not affected by the length of glaucoma before and after transplantation. Incorporation occurs less effectively after optic nerve transection, suggesting differing retinal environments after the two insults.
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