Purchase this article with an account.
Y. Mawatari, M. Fukushima, T. Inoue, T. Taga, H. Tanihara; Effects of Nonsteroidal Anti–Inflammatory Drug on Differentiation of Neural Progenitor Cells in the Diseased Retinas . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3248.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the effects of NSAID (nonsteroidal anti–inflammatory drug) on the differentiation of neural progenitor cells (NPCs) in the deseased retina. Methods: N–methyl–D–aspartate (NMDA; 4mM, 5ul) was injected into the vitreous space of adult rat to induce retinal cell death. NPCs were prepared from the telencephalic neuroepithelium of EGFP (enhanced green fluorescence protein) transgenic mice on embryonic day 14. Cell suspension (5x105 cells) was injected into vitreous space of NMDA–treat eyes. Immunohistochemical study and Western blot analysis were performed with or without intraperitoneal injection of indomethacin (2.5mg/kg, twice each day). Results: Western blot analysis showed that the expression of ciliary neurotrophic factor (CTNF), which induce glial differentiation of the progenitors, was significantly inhibited in NMDA–damage eye with NSAID treatments. Immunohistochemical study using antiserum against CNTF and glial fibrillary acidic protein (GFAP) revealed that the activation of host Müller glial cells after transplantation of NPCs was inhibited with the treatment of NSAID. Also, in transplanted NPCs with and without NSAID treatments, mean percentage of GFAP–positive (glial) cells was 27.5 ± 12.3 % and 63.5 ± 7.4 %, respectively.Conclusions:Our results showed inhibitory effects of NSAID against predominant glial differentiation of NPCs in the NMDA–treat retinas. It has been suggested that the NSAID treatments have beneficial effects on the retinal transplantation and subsequent regulation of NPCs for neuronal differentiation.
This PDF is available to Subscribers Only