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N. Yoshida, N. Katai, A. Sato, N. Yoshimura; Characterization of the Early Stage of Retinal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3259.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: In ARVO 2004, we presented that the retinal neovascularization developed through a multistep process. In this study, our purpose is to characterize the intra–retinal changes in the early stage of retinal neovascularization. Methods: To develop the retinal neovascularization, postnatal day 7 (P7) C57BL/6J mice were exposed to 75% oxygen for 5 days and then returned to room air. Antibodies against vascular endothelial growth factor (VEGF), Tie–2 and erythropoietin (Epo) were injected through the pars plana into mid–vitreous on P12. The control eye was injected with epuivalent volumes of mouse control IgG. On P13, fluorescein angiography that perfused through the left ventricle with FITC–dextran was performed and flat–mounted retinas were made. We assessed the migrated endothelial cells by counting CD34–positive cell number at the ischemic area. To confirm the factor associated with the early stage of retinal neovascularization, we also performed western blotting and RT–PCR by using the retinas from P12 to P15. Results: In the retina that injected Epo antibodies, the migratory cell number decreased significantly, but in other two factors, there were no significantly change after injection. In western blot analysis, it showed a time–dependent increase in Epo and Epo–receptor from P12 to P14. Conclusions: Epo may have a significant role for the migration process of endothelial cells in the early stage of retinal neovascularization.
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