May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Characterization of the Early Stage of Retinal Neovascularization
Author Affiliations & Notes
  • N. Yoshida
    Ophthalmology, Shinshu University, Matsumoto, Japan
  • N. Katai
    Ophthalmology, Shinshu University, Matsumoto, Japan
  • A. Sato
    Ophthalmology, Shinshu University, Matsumoto, Japan
  • N. Yoshimura
    Ophthalmology & Visual Science, Kyoto University, Kyoto, Japan
  • Footnotes
    Commercial Relationships  N. Yoshida, None; N. Katai, None; A. Sato, None; N. Yoshimura, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 3259. doi:
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    • Get Citation

      N. Yoshida, N. Katai, A. Sato, N. Yoshimura; Characterization of the Early Stage of Retinal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3259.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: In ARVO 2004, we presented that the retinal neovascularization developed through a multistep process. In this study, our purpose is to characterize the intra–retinal changes in the early stage of retinal neovascularization. Methods: To develop the retinal neovascularization, postnatal day 7 (P7) C57BL/6J mice were exposed to 75% oxygen for 5 days and then returned to room air. Antibodies against vascular endothelial growth factor (VEGF), Tie–2 and erythropoietin (Epo) were injected through the pars plana into mid–vitreous on P12. The control eye was injected with epuivalent volumes of mouse control IgG. On P13, fluorescein angiography that perfused through the left ventricle with FITC–dextran was performed and flat–mounted retinas were made. We assessed the migrated endothelial cells by counting CD34–positive cell number at the ischemic area. To confirm the factor associated with the early stage of retinal neovascularization, we also performed western blotting and RT–PCR by using the retinas from P12 to P15. Results: In the retina that injected Epo antibodies, the migratory cell number decreased significantly, but in other two factors, there were no significantly change after injection. In western blot analysis, it showed a time–dependent increase in Epo and Epo–receptor from P12 to P14. Conclusions: Epo may have a significant role for the migration process of endothelial cells in the early stage of retinal neovascularization.

Keywords: retinal neovascularization • ischemia • immunohistochemistry 
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