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S. Fickenscher, C. Rummelt, F.E. Kruse, W.L. Neuhuber, C. Cursiefen, F. Schroedl; Novel Expression and Characterization of Lymphatic Vessel Endothelial Hyaluronate Receptor (Lyve–1) in Normal Human Choroid . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3350.
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Purpose: Lymphatic vessel endothelial hyaluronic acid receptor (LYVE–1) is a newly discovered lymphatic endothelium–specific marker which is also expressed on a subpopulation of macrophages; to date, there is no report on its expression in the posterior uvea. Purpose of this study was to investigate the expression of LYVE–1 in normal human choroids. Methods:Meeting the Declaration of Helsinki, eyes of body–/cornea–donors (55–89 years of age; 4–9 hrs p.m.) were obtained from the Department of Anatomy or the Cornea Bank of the University of Erlangen. Choroids were dissected and prepared for cryo–sections followed by immunohistochemistry with anti–human LYVE–1 antiserum. Additionally, an anti–human CD–68 antibody was used. For documentation, light–, fluorescence– and confocal laser scanning microscopy were used. Results: The normal human choroidal stroma contains up to 248 LYVE–1 positive structures per square millimeter (mean). These structures displayed irregular shapes with a relatively uniform diameter of about 32 µm (mean). A nucleus (mean diameter: 12 µm) was often visible within the cytoplasm of these cells. Costaining with CD68 suggests, that these LYVE–1 immunoreactive cells most likely represent macrophages. Besides that, other fragmented, tubular LYVE–1 positive structures, morphologically not resembling macrophages, were discernible. The latter also do not resemble typical lymphatic vessels. Conclusions: The normal human choroid contains numerous LYVE–1 positive cells, presumably macrophages. These cells may be involved in choroidal hyaluronic acid metabolism or contribute to formation of lymphatic vessels under inflammatory conditions.
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