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D.E. Pelayes, J. Zarate, D. Colombero, C. Pastore, G. Piantoni; Cytologic Diagnosis of Intraocular Tumors With Monolayered Preparation . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3365.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the diagnostic utility of standarized monolayered preparation (AutoCyte preparation) from intraocular specimen obtained by fine needle aspiration biopsy or vitrectomy. Methods: Intraocular tumor samples from nine patients were obtained from fine needle aspiration biopsy in 8 cases and vitrectomy in two cases. Intraocular material was processed with monolayered preparations and stained with hematoxylin and eosin, PAS, Masson trichrome, and immunohistochemistry (HMB 45, Protein S100, vimentin and cytokeratine). Results: A correct diagnosis was achieved in all patients: choroidal melanoma in 6 cases, choroideal melanocytoma 1, iris melanoma 1, iris nevus 1 and nevus dysplasic of iris 1. Conclusions: The monolayered preparation technique is an accurate method to establish a cytologic diagnosis of intraocular tumors, particularly in cases where the samples are limited, and the clinical evaluation and ancillary tests are insufficient to make a diagnosis.
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