May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Cytologic Diagnosis of Intraocular Tumors With Monolayered Preparation
Author Affiliations & Notes
  • D.E. Pelayes
    Ophthalmology,
    University Buenos Aires, Buenos Aires, Argentina
    Pathology, Laboratory Investigation Ophthalmologic and Visual Science (LIOCiV), University of Buenos Aires, Argentina
  • J. Zarate
    Pathology,
    University Buenos Aires, Buenos Aires, Argentina
    Pathology, Laboratory Investigation Ophthalmologic and Visual Science (LIOCiV), University of Buenos Aires, Argentina
  • D. Colombero
    Ophthalmolgy, University of Buenos Aires, Rosario, Argentina
  • C. Pastore
    Ophthalmology,
    University Buenos Aires, Buenos Aires, Argentina
  • G. Piantoni
    Ophthalmology,
    University Buenos Aires, Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships  D.E. Pelayes, None; J. Zarate, None; D. Colombero, None; C. Pastore, None; G. Piantoni, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 3365. doi:
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    • Get Citation

      D.E. Pelayes, J. Zarate, D. Colombero, C. Pastore, G. Piantoni; Cytologic Diagnosis of Intraocular Tumors With Monolayered Preparation . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3365.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate the diagnostic utility of standarized monolayered preparation (AutoCyte preparation) from intraocular specimen obtained by fine needle aspiration biopsy or vitrectomy. Methods: Intraocular tumor samples from nine patients were obtained from fine needle aspiration biopsy in 8 cases and vitrectomy in two cases. Intraocular material was processed with monolayered preparations and stained with hematoxylin and eosin, PAS, Masson trichrome, and immunohistochemistry (HMB 45, Protein S100, vimentin and cytokeratine). Results: A correct diagnosis was achieved in all patients: choroidal melanoma in 6 cases, choroideal melanocytoma 1, iris melanoma 1, iris nevus 1 and nevus dysplasic of iris 1. Conclusions: The monolayered preparation technique is an accurate method to establish a cytologic diagnosis of intraocular tumors, particularly in cases where the samples are limited, and the clinical evaluation and ancillary tests are insufficient to make a diagnosis.

Keywords: tumors • pathology techniques • melanoma 
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