Purchase this article with an account.
M. Suzuki, M. Kamei, J.G. Hollyfield, K. Yoneda, H. Itabe, H. Sakaguchi, Y. Tano; Oxidized Low Density Lipoprotein in Human Retinal Pigment Epithelium Cells and Photoreceptor Outer Segments Increase With Age . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3504.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: Age–related macular degeneration (AMD) might occur via a mechanism similar to atherosclerosis, in which uptake of oxidized low density lipoprotein (Ox–LDL) by macrophages via scavenger receptors involves the initial lesion. We previously showed that Ox–LDL and scavenger receptors are also present in AMD. We therefore hypothesized that Ox–LDL may be a key molecule in AMD as it is in atherosclerosis. However, there is no report on the distribution of Ox–LDL in AMD eyes and even in normal eyes. In this study, we examined the distribution of Ox–LDL in normal eyes, investigated changes with age, and also compared AMD eyes and age–matched normal eyes. Methods: Donor tissues analyzed included 20 normal eyes (22–98 years old) and 2 AMD eyes (68 and 70 years old). A tissue strip including the fovea was used for immunohistochemistry, which was performed with DLH3, anti–Ox–LDL antibody. The intensity of the immunostaining was graded as none (–), mild (+), moderate (++), heavy (+++), or very heavy (++++). Results: Positive staining for Ox–LDL was observed in the photoreceptor outer segments (POS) and retinal pigment epithelium (RPE) in normal human eyes. The POS demonstrated diffuse immunoreactivity with prominent staining at its tip. RPE showed a weaker immunoreactivity for Ox–LDL compared to the POS. Eyes from older donors showed more intense immunostaining than eyes from younger donors both in the POS and RPE. AMD eyes showed more intense immunoreactivity compared with age–matched normal eyes, which was more prominent in the RPE than in the POS. Ox–LDL immunoreactivity was also present in the soft drusen in AMD eyes. Conclusions: We found that Ox–LDL is present in normal human eyes, and the immunoreactivity increases with age. In addition, AMD eyes show more intense immunoreactivity compared with age–matched normal eyes. These results show a possible link between Ox–LDL and AMD, and promote the hypothesis that oxidative damage to the POS and RPE may be a potential pathway for AMD.
This PDF is available to Subscribers Only