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W.–B. Liu, Q. Yan, H. Feng, X.–Q. Huang, Y. Liu, M. Deng, S.–M. Sun, L. Gong, J.–P. Liu, D.W. Li; Differential Expression of the Protein Phosphatase–1, and –2A in the Eye and Other Tissues of Gold Fish . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3524.
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Purpose: Protein phosphatase–1 (PP–1) and –2A (PP–2A) are major cellular serine/threonine phosphatases. We have previously shown that both PP–1 and PP–2A are mainly expressed in the epithelial cells of the ocular lens from bovine and rat (Li et al., 2001. IOVS, 42:2603–2609). In the present study, we have further examined the expression patterns of the two phosphatases in the different compartments of the eye and also other tissues of goldfish, an excellent organism for studying eye development. Methods: Different compartments of the eye and various other tissues were isolated for extraction of total proteins. Western blot was used to analyze the relative levels of the catalytic subunits for PP–1 and PP–2A in the above tissues. Results: In the different compartments of the eye, the highest level of PP–1 expression is found in the retina. Among the 14 different tissues examined, retina, kidney, brain, ovary, testis and gill display highest level of PP–1 expression. In contrast, PP–2A was found highly expressed in liver, pancreas, brain, ovary and gill. In the different compartments of the eye, only very level of PP–2A was detected. Conclusions: PP–1 is a major phosphatase in the eye of goldfish. Supported by the Hormel Foundation and Lotus Scholar Professorship Funds from Hunan Province Government and Hunan Normal University. None.
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