May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Map Kinases Regulate Eye Development in Goldfish
X. Huang; Y. Liu; H. Feng; W.–B. Liu; Q. Yan; C. Luo; S.–J. Liu; X.–J. Zhang; Y. Liu; D.W. Li
Author Affiliations & Notes
  • X. Huang
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • Y. Liu
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • H. Feng
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • W.–B. Liu
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • Q. Yan
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • C. Luo
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • S.–J. Liu
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • X.–J. Zhang
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • Y. Liu
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
  • D.W. Li
    College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
    The Hormel Institute, University of Minnesota, Austin, MN
  • Footnotes
    Commercial Relationships  X. Huang, None; Y. Liu, None; H. Feng, None; W. Liu, None; Q. Yan, None; C. Luo, None; S. Liu, None; X. Zhang, None; Y. Liu, None; D.W. Li, None.
  • Footnotes
    Support  The Hormel Foundation and the Lotus Scholar Professorship Funds
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 3526. doi:
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      X. Huang, Y. Liu, H. Feng, W.–B. Liu, Q. Yan, C. Luo, S.–J. Liu, X.–J. Zhang, Y. Liu, D.W. Li; Map Kinases Regulate Eye Development in Goldfish . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3526.

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Abstract

Abstract: : Purpose:Our previous studies have revealed that MAP kinases are highly expressed in the ocular lens of human, bovine and rat eyes (Li et al., 2003. IOVS. 44:5277–5286). In the present study, we have used goldfish as a model organism to explore the functions of MAP kinases, JNK and ERK, in regulating eye development. Methods:Both diploid and hyploid goldfish larvae were produced and verified through phenotype analysis. Expression and activities of JNK and ERK in diploid and hyploid goldfish larvae were analyzed by Western blot. Dominant negative mutant JNK and ERK were introduced into fertilized eggs, or artificially activated eggs and their interferences on eye development of goldfish were examined by immunocytochemistry, histology and morphology. Results: In hyploid goldfish with eye abnormality, expression of JNK1 was almost suppressed and expression of ERK1/2 were substantially enhanced. Dominant negative JNK and ERK mutants interfere with normal activity of JNK and ERK, and clearly disturb the normal development of goldfish eye. Conclusions:MAP kinases, especially JNK, play an important role in eye development. Supported by the Hormel Foundation and the Lotus Scholar Professorship Funds from Hunan Province Government and Hunan Normal University. None

Keywords: visual development • gene/expression • phosphorylation 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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