Purchase this article with an account.
X. Huang, Y. Liu, H. Feng, W.–B. Liu, Q. Yan, C. Luo, S.–J. Liu, X.–J. Zhang, Y. Liu, D.W. Li; Map Kinases Regulate Eye Development in Goldfish . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3526.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose:Our previous studies have revealed that MAP kinases are highly expressed in the ocular lens of human, bovine and rat eyes (Li et al., 2003. IOVS. 44:5277–5286). In the present study, we have used goldfish as a model organism to explore the functions of MAP kinases, JNK and ERK, in regulating eye development. Methods:Both diploid and hyploid goldfish larvae were produced and verified through phenotype analysis. Expression and activities of JNK and ERK in diploid and hyploid goldfish larvae were analyzed by Western blot. Dominant negative mutant JNK and ERK were introduced into fertilized eggs, or artificially activated eggs and their interferences on eye development of goldfish were examined by immunocytochemistry, histology and morphology. Results: In hyploid goldfish with eye abnormality, expression of JNK1 was almost suppressed and expression of ERK1/2 were substantially enhanced. Dominant negative JNK and ERK mutants interfere with normal activity of JNK and ERK, and clearly disturb the normal development of goldfish eye. Conclusions:MAP kinases, especially JNK, play an important role in eye development. Supported by the Hormel Foundation and the Lotus Scholar Professorship Funds from Hunan Province Government and Hunan Normal University. None
This PDF is available to Subscribers Only