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S. Biswas, P. Bhattacherjee, C.A. Paterson, T. Maruyama, S. Narumiya; EP1 Receptors and Intraocular Inflammation . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3667.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the role of prostaglandin E2 receptor subtype EP1 in intraocular inflammation. Methods: Inflammation was induced in the EP1 receptor wild type (+/+) and EP1 receptor knockout (–/–) mice eyes, by topical administration of PGE2 or butaprost or by intravitreal injection of LPS or by paracentesis. A separate group of wild type mice were treated with ONO–8713 (EP1 receptor specific antagonist) prior to treatment with the agonist. Thirty five to 40 minutes after PGs or paracentesis and 24 hours after LPS, aqueous humor protein levels were determined and leukocytes counted. Results: There was a significant increase in the aqueous humor protein values in the EP1 receptor –/– mice after PGE2, butaprost, paracentesis and LPS treatments. The protein value in PGE2 treated (+/+) mouse was 1.0 mg/ml whereas that in (–/–) mouse was 7.2 mg/ml. In butaprost treated (+/+) mouse the protein value was 1.73 mg/ml and that of (–/–) mouse was 7.0 mg/ml. In LPS and paracentesis, the protein values in (+/+) mouse were 24–26 mg /ml whereas the (–/–) mouse protein values were around 40 mg/ml. Leukocute counts were over 50% higher in the LPS treated (–/–) mouse as compared to the (+/+) mouse. Conclusions: The above results suggest that EP1 receptors play a counter inflammatory role in experimentally induced ocular inflammation.
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