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C. Law, C.Y. Chan, O.A. Candia, C.–H.H. To; Fluid Transport Across Porcine Ciliary Body Epithelium . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3682.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To measure the rate of fluid transport across the porcine ciliary body epithelium (CBE) using a modified Ussing–type chamber. Methods: A whole ring of porcine CBE was excised and mounted in a modified Ussing–type chamber. Ringer reservoir with bubbling system was positioned on one hemi–chamber, and a graduated capillary tube on the other hemi–chamber. Changes of the capillary level reflected the fluid formation rate of the CBE. The potential difference (PD) across the CBE was continuously monitored. The effects of ouabain on both PD and fluid formation rate were examined. Results: The rate of fluid transport across the CBE was 2.13 ± 0.14 µl/hr/cm2 (n=12). PD across the CBE was 1.25 ± 0.16 mV (n=12), with the aqueous side consistently negative. Stromal addition of ouabain caused biphasic response of PD, and significantly reduced the fluid transport across the CBE by 66%. Conclusions: The fluid formation across the porcine CBE was active and responsive to the stromal addition of ouabain.
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