May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Fluid Transport Across Porcine Ciliary Body Epithelium
C. Law; C.Y. Chan; O.A. Candia; C.–H.H. To
Author Affiliations & Notes
  • C. Law
    Department of Optometry and Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • C.Y. Chan
    Department of Optometry and Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • O.A. Candia
    Department of Ophthalmology, Mount Sinai School of Medicine, New York, NY
  • C.–H.H. To
    Department of Optometry and Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • Footnotes
    Commercial Relationships  C. Law, None; C.Y. Chan, None; O.A. Candia, None; C.H. To, None.
  • Footnotes
    Support  NEI Grant EY013749–02, USA, and Central Research Grants 8–ZF84 and G–T807 from HKPU
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 3682. doi:
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      C. Law, C.Y. Chan, O.A. Candia, C.–H.H. To; Fluid Transport Across Porcine Ciliary Body Epithelium . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3682.

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Abstract

Abstract: : Purpose: To measure the rate of fluid transport across the porcine ciliary body epithelium (CBE) using a modified Ussing–type chamber. Methods: A whole ring of porcine CBE was excised and mounted in a modified Ussing–type chamber. Ringer reservoir with bubbling system was positioned on one hemi–chamber, and a graduated capillary tube on the other hemi–chamber. Changes of the capillary level reflected the fluid formation rate of the CBE. The potential difference (PD) across the CBE was continuously monitored. The effects of ouabain on both PD and fluid formation rate were examined. Results: The rate of fluid transport across the CBE was 2.13 ± 0.14 µl/hr/cm2 (n=12). PD across the CBE was 1.25 ± 0.16 mV (n=12), with the aqueous side consistently negative. Stromal addition of ouabain caused biphasic response of PD, and significantly reduced the fluid transport across the CBE by 66%. Conclusions: The fluid formation across the porcine CBE was active and responsive to the stromal addition of ouabain.

Keywords: aqueous • ciliary body 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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