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N.A. Sharif, C.R. Kelly, J.Y. Crider, M. Senchyna; RT–PCR Mapping of Serotonin Receptor Subtype mRNAs in Human Ciliary Body and Trabecular Meshwork . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3688.
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To study the relative distribution of the mRNAs for serotonin (5HT) receptor subtypes in human ocular tissues / cells using reverse transcriptase polymerase chain reaction (RT–PCR) and determine receptor–mediated functional responses in isolated human ocular cells. Standard RT–PCR procedures were utilized for the mRNA detection (up to 10 human donor eyes for tissues; n = 8 donor human trabecular meshwork (h–TM) cell samples). Phosphoinositide (PI) hydrolysis and intracellular Ca2+ ([Ca2+]i ) mobilization were determined using previously published methods.
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