Abstract
Abstract: :
Purpose: Versican, chondroitin sulfate glycoprotein 2, is thought to play a role in regulating aqueous humor outflow and intraocular pressure via the human trabecular meshwork (HTM) of the eye. This protein was upregulated in HTM cells when they were treated with TGF–beta. There are four splice variant forms of versican (V0–V3) with different numbers of glycoaminoglycan (GAG) attachment domains. In this study, we investigated the various isoforms of versican from ocular tissues and cultured cells. Methods: HTM and HCM (human ciliary muscle) tissues were dissected from three pairs of donors eyes with no histories of eye diseases. Cultures of HTM and HCM cells were established from five donor eyes, and HTM cells were treated for 72 hours with 1 ng/ml of either with hrTGF–beta1 or hrTGF–beta2. Total RNA were isolated from cells and tissues from each of the samples. Relative quantitation of gene expression of each variant was detected by real–time PCR with SYBR Green dye. Results: All four variants of versican existed in each sample. In cultured HTM cells, the two variants with the largest number of GAG domains predominate. The V1 form is about 20% greater than the V0 form. There is an upregulation, particularly in the V0 form, when the cells are cultured. In tissue, the V1 form is about five fold greater than the other three. In the ocular ciliary muscle, the V1 form is the most prominent, but with this tissue, the relative amount of the V0 form did not change when cell were cultured. The increases in expression in the HTM with TGF–beta treatment were greater with the V0 and V2 forms. Conclusions: This is the first report about the presence of various forms of versican in the anterior segment of the eye and the alterations in the mRNA patterns of these forms when cells are placed in culture. The results indicate the variants with the largest numbers of GAG attachment domains are the most prominent in the HTM and HCM.
Keywords: trabecular meshwork • gene/expression