May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Differences in Retinal Oxygen Saturation in Rat Measured With Hyperspectral Imaging
J.–X. Qian; H.A. Leder; J. Ning; L. Andara; J.M. Beach; B. Khoobehi
Author Affiliations & Notes
  • J.–X. Qian
    Ophthalmology, Louisiana State Univ Hlth SciCtr, New Orleans, LA
  • H.A. Leder
    Ophthalmology, Louisiana State Univ Hlth SciCtr, New Orleans, LA
  • J. Ning
    Ophthalmology, Louisiana State Univ Hlth SciCtr, New Orleans, LA
  • L. Andara
    Biological Sciences, University of New Orleans, New Orleans, LA
  • J.M. Beach
    Ophthalmology, Louisiana State Univ Hlth SciCtr, New Orleans, LA
    Institute for Technology Development, Stennis Space Center, MS
  • B. Khoobehi
    Ophthalmology, Louisiana State Univ Hlth SciCtr, New Orleans, LA
  • Footnotes
    Commercial Relationships  J. Qian, None; H.A. Leder, None; J. Ning, None; L. Andara, None; J.M. Beach, None; B. Khoobehi, None.
  • Footnotes
    Support  NIH P30EY002377 (core grant); RPB
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 3911. doi:
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      J.–X. Qian, H.A. Leder, J. Ning, L. Andara, J.M. Beach, B. Khoobehi; Differences in Retinal Oxygen Saturation in Rat Measured With Hyperspectral Imaging . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3911.

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Abstract

Abstract: : Purpose: To employ hyperspectral imaging technology as a means of non–invasive evaluation of the oxygen saturation of retinal vessels and tissues in normal rats. Methods: The mean value of the relative oxygen saturation index (RSI) of retinal arteries and veins, as well as retinal tissue (microcirculation), were measured using retinal hyperspectral images based on the difference in reflectance spectra between oxygenated hemoglobin and reduced hemoglobin. Oxygen saturation maps were generated from hyperspectral data with MATLAB. Results:The results showed clear differences in oxygen saturation in the main arteries and veins. Differences in oxygen saturation between regions of retinal tissue were statistically significant. In terms of mean RSI values, differences between the peripapillary area (0.047 ± 0.012), the mid retina (0.040 ± 0.006), and the peripheral retina (0.042 ± 0.009) were significant (P = 0.002). The mean RSI value for the temporal side of the optic nerve head (0.046 ± 0.010) was significantly higher than that for the nasal retinal area (0.040 ± 0.008; P < 0.001). Oxygen saturation maps transformed from the hyperspectral images demonstrated low to high oxygen saturation distribution for retinal tissue and vessels. Conclusions: This experiment suggests that hyperspectral imaging can provide non–invasive retinal oxygen saturation measurements in animals such as rats.

Keywords: imaging/image analysis: non-clinical • retina • blood supply 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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