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J.–X. Qian, H.A. Leder, J. Ning, L. Andara, J.M. Beach, B. Khoobehi; Differences in Retinal Oxygen Saturation in Rat Measured With Hyperspectral Imaging . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3911.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To employ hyperspectral imaging technology as a means of non–invasive evaluation of the oxygen saturation of retinal vessels and tissues in normal rats. Methods: The mean value of the relative oxygen saturation index (RSI) of retinal arteries and veins, as well as retinal tissue (microcirculation), were measured using retinal hyperspectral images based on the difference in reflectance spectra between oxygenated hemoglobin and reduced hemoglobin. Oxygen saturation maps were generated from hyperspectral data with MATLAB. Results:The results showed clear differences in oxygen saturation in the main arteries and veins. Differences in oxygen saturation between regions of retinal tissue were statistically significant. In terms of mean RSI values, differences between the peripapillary area (0.047 ± 0.012), the mid retina (0.040 ± 0.006), and the peripheral retina (0.042 ± 0.009) were significant (P = 0.002). The mean RSI value for the temporal side of the optic nerve head (0.046 ± 0.010) was significantly higher than that for the nasal retinal area (0.040 ± 0.008; P < 0.001). Oxygen saturation maps transformed from the hyperspectral images demonstrated low to high oxygen saturation distribution for retinal tissue and vessels. Conclusions: This experiment suggests that hyperspectral imaging can provide non–invasive retinal oxygen saturation measurements in animals such as rats.
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