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Y. Hayashi, R. Ohtani–Kaneko, Y. Munemasa, K. Kuribayashi, Y. Kitaoka, J. Kogo, K. Hirata, S. Ueno; Neuroprotective Effects of 17ß–estradiol on the Rat Retina Exposed to NMDA Administration . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4001.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To examine the neuroprotective effect of 17ß–estradiol on the rat retina exposed to N–methyl–D–aspartate (NMDA) administration. Methods: Eight–week–old female Wistar rats were used as subjects. All of the rats were divided into sham operated and ovariectomized (OVX) groups. The OVX rats were treated either with solvent vehicle or 17ß–estradiol subcutaneous implant. At 1 week post–operation, all rats were received intravitreal injection of 100 nmol NMDA into the one eye, and the same amount of phosphate buffered saline (PBS) into the another eye. The rats were fixed by pericardial perfusion with 4% paraformaldehyde in 0.1 M phosphate buffer (PB), and eyes were enucleated at 12, 24 hours and 7days after injection. The retinas were embedded in paraffin. Then, thin sections were cut and stained with Hematoxylin and Eosin to examine the number of retinal ganglion cells (RGCs) and the thickness of the inner nuclear layer (INL). TUNEL staining was also applied to examine the localization of apoptotic cells. Results: Twelve hours after NMDA injection, the number of TUNEL–positive cells in the INL was significantly decreased by 17ß–estradiol implant, compared with solvent vehicle implant. Seven days after NMDA injection, NMDA–induced decrease in thickness of the IPL and the INL had a tendency to be suppressed by 17ß–estradiol implant. Conclusions: Studies on NMDA–induced apoptosis in RGCs as well as morphometric studies on the thickness of the IPL and INL indicated the neuroprotective effect of 17ß–estradiol on the inner retina.
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