Cullin–5 is Associated With Retinal Ganglion Cell (RGC) Death in Glaucoma Model Mice

Abstract

Abstract: : Purpose: To investigate the role of Cullin–5 in RGC death, as the target from proteomics of DBA/2J mice. Methods: Proteomics analysis was performed using whole retinal samples, lysed from DBA/2J mice at the age of five, seven, and eleven months. Upregulated spots, including Cullin–5, were identified by mass spectrometry. The change of Cullin–5 expression was also confirmed by western blotting. To check the localization of Cullin–5, mouse (C57BL) retina was immunostained with specific anti–Cullin–5 antibody (Santa Cruz). Results: Proteomics and mass spectrometry revealed that Cullin–5 expression dramatically increased at seven months between five and eleven months. Immunohistochemical analysis of mouse retina showed Cullin–5 localized in the inner retina, especially in RGCs. Conclusions: Cullin–5 may have an important role in RGC death, and that will be contributed to clarify the mechanism of glaucoma.

Keywords: ganglion cells • retinal degenerations: cell biology • proteomics