May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Synthetic Bruch's Membrane Substitutes: Comparisons After Subretinal Transplantation With Cultured Iris Pigment Epithelium
F.E. Molnar; L. Lombardi; N. Berker; E. Yeh; D. Yellachich; T. Leng; R. Dalal; M.F. Marmor; H.A. Fishman; M.S. Blumenkranz
Author Affiliations & Notes
  • F.E. Molnar
    Department of Ophthalmology, Stanford University, Stanford, CA
  • L. Lombardi
    Department of Ophthalmology, Stanford University, Stanford, CA
  • N. Berker
    Department of Ophthalmology, Stanford University, Stanford, CA
  • E. Yeh
    Department of Ophthalmology, Stanford University, Stanford, CA
  • D. Yellachich
    Department of Ophthalmology, Stanford University, Stanford, CA
  • T. Leng
    Department of Ophthalmology, Stanford University, Stanford, CA
  • R. Dalal
    Department of Ophthalmology, Stanford University, Stanford, CA
  • M.F. Marmor
    Department of Ophthalmology, Stanford University, Stanford, CA
  • H.A. Fishman
    Department of Ophthalmology, Stanford University, Stanford, CA
  • M.S. Blumenkranz
    Department of Ophthalmology, Stanford University, Stanford, CA
  • Footnotes
    Commercial Relationships  F.E. Molnar, VISX, Inc. P; L. Lombardi, VISX, Inc. P; N. Berker, VISX, Inc. P; E. Yeh, VISX, Inc. P; D. Yellachich, VISX, Inc. P; T. Leng, VISX, Inc. P; R. Dalal, VISX, Inc. P; M.F. Marmor, VISX, Inc. P; H.A. Fishman, VISX, Inc. P; M.S. Blumenkranz, VISX, Inc. P.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4154. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Synthetic Bruch's Membrane Substitutes: Comparisons After Subretinal Transplantation With Cultured Iris Pigment Epithelium
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      F.E. Molnar, L. Lombardi, N. Berker, E. Yeh, D. Yellachich, T. Leng, R. Dalal, M.F. Marmor, H.A. Fishman, M.S. Blumenkranz; Synthetic Bruch's Membrane Substitutes: Comparisons After Subretinal Transplantation With Cultured Iris Pigment Epithelium . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4154.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: Transplantation of retinal (RPE) or iris pigment epithelial cells (IPE) on artificial substrates could be beneficial for age related macular degeneration, since the aged Bruch's membrane may no longer be an adequate surface. We explore the use of polycarbonate (PC) and cellulose ester (CE) membranes for the implantation of cultured iris pigment epithelial cells. Methods: IPE cells were cultured on PC or CE membranes with 10 µm thickness. Autologous IPE cells were harvested by iridectomy from New Zealand red rabbits and seeded on membranes. The cultured IPE cells were analyzed by light microscopy, live/dead fluorescent staining, and immunohistochemistry. Seven membranes of each type, with confluent IPE monolayer, were implanted subretinally by raising a bleb with balanced salt solution for insertion. Eyes were removed and histological changes were analyzed at 1 and 3 months. Results: Cultures of IPE grew as monolayers on both membranes, but histochemistry showed fibrotic rather than epitheloid morphology and a loss of pigment granules. Histology of implanted membranes at both 1 and 3 months showed no inflammation, but varying degrees of fibrous proliferation above the membranes. Few IPE cells were identifiable. RPE migration was observed around the ends of the membranes. The overlying retina showed a loss of outer segments and outer nuclear layer disruption, but inner retinal layers were preserved. There was no clear difference between PC and CE, except that PC was easier to implant. Conclusions: PC and CE membranes can be implanted subretinally without rejection, although PC is easier to manipulate. IPE cultured on these substrates showed poor epithelial preservation and varying amounts of fibrous proliferation when implanted. The concept of Bruch's membrane replacement is promising, but IPE transplantation remains fraught with difficulties.

Keywords: transplantation • retinal pigment epithelium • age-related macular degeneration 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept