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Y. Gohto, A. Obana, T. Hirano, E. Kohno, E. Oishi, Y. Nagase; Pigment Epithelium–Derived Factor and Basic Fibroblast Growth Factor Production by the Retinal Pigment Epithelial Cells After Hyperthermia . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4175.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Transpupillary thermotherapy (TTT) induces occlusion of choroidal neovascularization, however, the mechanisms of therapeutic effect are still controversial. Heat induces necrosis of the vascular wall cells, while some cytokines such as heat shock protein is speculated to induce stabilization of the neovascularization. In this study, the production of pigment epithelium–derived factor (PEDF), basic fibroblast growth factor (basic FGF), and vascular endothelial growth factor(VEGF) after thermo stress was examined on the retinal pigment epithelial cells(RPE cells) and vascular endothelial cells. Methods: Cultured human RPE cells (ARPE–19, ATCC) and vascular endothelial cells of monkey choroid and retina (CRVEC, ATCC) were used. The cells were incubated in an incubator equilibrated 37, 42 and 47 oC for 5 and 20 minutes as a heat–shock procedure. One and three days after the procedure, cell viability was determined by MTS assay(CellTiter96). PEDF, basic FGF, and VEGF were measured by immunoassay (Chemikine PEDF ELISA Kit, Quantikine, AN’ALYZA) in cells and medium. Results: The cell viability of both cells was nearly 100%, after 42oC incubation. In the case of 47oC and 20 min incubation, the cell viability was less than 50%. After 47oC and 20 min incubation, PEDF in the RPE cells and basic FGF in the RPE medium were 6 and 50 times higher than the controls(incubated at 37 oC), respectively. VEGF in the RPE medium decreased after incubation at 47oC, while VEGF in the CRVEC increased. Conclusions: The release of cytokines such as PEDF and basic FGF and reduction of VEGF may be responsible for the therapeutic effect of hyperthermia to choroidal neovascularization.
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