Effect of the Endothelial Progenitor Cell on the Angiogenesis and Vasculogenesis of the Anophthalmic Socket Implant

Abstract

Abstract: : Purpose: The recent discovery of endothelial progenitor cells(EPCs) in adult blood has demonstrated that the growth of new blood vessels not only cccurs by the sprouting of pewexisting vessels but also involves cells that are able to circulate and differenciate in situ. Circulating EPCs would selectively localize to regions of safe tissue ischemia, induce neovascularization and increase tissue survival. Methods: The EPCs were cultured according to previously published techniques, in full accordance with the New York University Institutional Review Board Guidelines.The Medpor processed with EPCs , with the human microvascular endothelial cell and only with culture media were implanted into the abdominal wall of athymic nude mice. Left abdominal wall has covered with dermis and right abdominal wall exposed for 4 weeks. We evaluate the degree of neovascularization of orbital implants. Results: By postoperative day 7, the exposed Medpor s processed with the EPCs shows more advanced blood vessel ingrowth than other groups. The regions of survival and necrosis were clearly demarcated in covered flaps. Identification of EPCs was confirmed by both fluorescent microscopy and immunohistochemistry. Capillary density was significantly greater by day 14 in athymic nude mice transplanted with EPCs. Conclusions: The delivery of EPCs immediately after surgery have resulted in a limited ability of EPCs to prevent flap necrosis. Because significant improvements in neovascularization were not seen until 14 days after injection, it is not surprising that EPCs transplantation immediately afer the ischemic event did not results in increased survival. Delivering EPCs before ischemia of Medpor exposure area may potentially be more successful. Application of EPCs promote the blood vessel growth into the pore of Medpor and prevents the orbital implant infection

Keywords: wound healing