May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Genetic Mapping of Choroidal Neovascularization (CNV) Following Laser Photocoagulation in Inbred Strains of Mice
T. Funakoshi; T. Baba; A.E. Birsner; R.J. D'Amato
Author Affiliations & Notes
  • T. Funakoshi
    Vascular Biology Program, Childrens Hospital Boston, Boston, MA
  • T. Baba
    Division of Ophthalmology and Visual Science, Department of Medicine of Sensory and Motor Organs, Faculty of Medicine, Tottori University, Yonago, Japan
  • A.E. Birsner
    Vascular Biology Program, Childrens Hospital Boston, Boston, MA
  • R.J. D'Amato
    Vascular Biology Program, Childrens Hospital Boston, Boston, MA
    Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Footnotes
    Commercial Relationships  T. Funakoshi, None; T. Baba, None; A.E. Birsner, None; R.J. D'Amato, None.
  • Footnotes
    Support  RPB Lew Wasserman Merit award and NIH Grant R01–EY12726
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4190. doi:
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      T. Funakoshi, T. Baba, A.E. Birsner, R.J. D'Amato; Genetic Mapping of Choroidal Neovascularization (CNV) Following Laser Photocoagulation in Inbred Strains of Mice . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4190.

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Abstract

Abstract: : Purpose: To perform genetically mapping of the differences in laser induced choroidal neovascularization (CNV) among various recombinant inbred (RI) strains. Methods: We utilized a laser induced murine CNV model. C57BL/6J, DBA/2J, 19 BXD recombinant inbred strains (8 black and 11 steel gray coat) derived from C57BL/6J and DBA/2J , and 129S1/SvImJ mice were used for this study. 2 weeks after laser treatment, digital images of CNV were obtained from fluorescein isothiocyanate–dextran (FITC–dextran) angiography, choroidal flat mount and histological examination. These were quantified by NIH image software on PC. Results: RI mice with different levels of pigmentation were grouped together (since laser photocoagulation can be altered by pigmentation). Within each group, there were significant differences in CNV size across RI strains. The data from FITC–dextran angiography and choroidal flat mount were correlated. Conclusions: FITC–dextran angiography, choroidal flat mount and histological examination were useful for evaluating laser induced murine CNV model. Genetic mapping of the loci that influence CNV in RI strains will be presented.

Keywords: age-related macular degeneration • choroid: neovascularization • laser 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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