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T. Funakoshi, T. Baba, A.E. Birsner, R.J. D'Amato; Genetic Mapping of Choroidal Neovascularization (CNV) Following Laser Photocoagulation in Inbred Strains of Mice . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4190.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To perform genetically mapping of the differences in laser induced choroidal neovascularization (CNV) among various recombinant inbred (RI) strains. Methods: We utilized a laser induced murine CNV model. C57BL/6J, DBA/2J, 19 BXD recombinant inbred strains (8 black and 11 steel gray coat) derived from C57BL/6J and DBA/2J , and 129S1/SvImJ mice were used for this study. 2 weeks after laser treatment, digital images of CNV were obtained from fluorescein isothiocyanate–dextran (FITC–dextran) angiography, choroidal flat mount and histological examination. These were quantified by NIH image software on PC. Results: RI mice with different levels of pigmentation were grouped together (since laser photocoagulation can be altered by pigmentation). Within each group, there were significant differences in CNV size across RI strains. The data from FITC–dextran angiography and choroidal flat mount were correlated. Conclusions: FITC–dextran angiography, choroidal flat mount and histological examination were useful for evaluating laser induced murine CNV model. Genetic mapping of the loci that influence CNV in RI strains will be presented.
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