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X. Zhao, S. Briggs, G. Belovay, F. Altomare, S.R. Boyd; Endothelial Precursor Cells Regulate the Cellular Phenotype of Retinal Stem Cells . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4198.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the influence of endothelial precursor cells (EPCs) on the behaviour of retinal stem cells (RSCs). Methods: EPCs were isolated from the bone marrow, and RSCs from the ciliary body, of CD1 mice. Indirect and direct co–culture techniques were used. By indirect co–culture, RSC colonies were grown separated from EPCs by a membranous partition that prevents cell contact but permits soluble factors to pass. In direct co–culture, RSCs and EPCs were grown together on a common substrate. Various substrates and media were used to provide permissive or non–permissive conditions. Direct microscopy and immunohistochemistry were used to assess cell morphology, migration and differentiation. Results:EPCs are able to rescue RSCs grow on a non–permissive substrate. EPCs consistently result in higher rates of RSC migration and greater expansion of colonies. The differentiation of RSCs along the glial lineage appears to be altered by the presence of EPCs, and we are currently addressing their effect on the neuronal lineage. Reciprocally, it also appears that RSCs alter EPC phenotype. In direct co–culture, neurally–derived nestin–positive strands were observed to align alongside forming endothelial tubes. Conclusions: EPCs influence RSC development and differentiation through a paracrine exchange of signals. We are currently quantifying and expanding these observations, and evaluating at least two signaling pathways we believe to be involved. Understanding this molecular dialogue will provide insights into neurogenesis and its relationship with angiogenesis.
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