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M. Yan, M. Zhang, R.A. Adelman, J. Zhang, Y. Liu, F. Lu; Prediction of Inhibitive Activity of Endostatin in vitro by Gene Expression Profiling . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4199.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To predict the inhibitive activity on retianl microvascular endothelial cells of endostatin by gene expression profiling. To predict the inhibitive activity on retianl microvascular endothelial cells of endostatin by gene expression profiling. Methods: Human retianl microvascular endothelial cells were cultured with 2500ng/ml endostatin for 24h, then the total RNA was extracted and analyzed by hybridized with BiostarH–40S gene expression DNA microarrays. Results: Approximately 5.96% of all human genes examined on 4096 gene microarrays exhibited altered expression, with 5.49% showing down–regulation and 0.47% showing up–regulation. Endostatin down–regulated genes encoding for cell proliferation, migration and differentiation. Conclusions: Down–regulation of those genes by endostatin may result in a potent antiangiogenesis effect.
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