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B.–Y. Ahn, W. You, O.–W. Kwon, D.–S. Kim; The Pro–Angiogenic Activity of EGT001 Promotes the Vessel Stabilization by Angiopoietin–1 . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4202.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: A number of reports have been reported therapeutic angiogenesis using Angiopoietin–1 (ANG1), because ANG1 has recruits and sustains peri–endothelial support cells, while Angiopoietin–2 (ANG2) disrupts blood vessel formation. Even ANG1 may provide a therapeutic benefit for cure of abnormal neovascularization, ANG1 is not easily use caused by poor solubility and stability. This study was aimed to investigate the pro–angiogenic effect of EGT001, which could induce the ANG 1. Methods: The effects of EGT001 were examined in colloidal nenovascularization (CNV) model in the rabbit. The vessel formation of retina was observed by angiography. Retinal tissues was stained with hematoxilin and eosin (H&E), and observed under a light microscope. To evaluate the ANG1 inducing activity of EGT001, it was intraperitoneally administrated as a 1ug/kg per mouse. On postoperative 12 hrs after treatment, the expression level of ANG1 in each organ, such as liver, eye, spleen and blood, was determined by western blotting. Results: In the control group, the laser induced colloidal neovascularization, however the administration of EGT001 (10 ug/kg) significantly inhibited abnormal neovascularization compared with control group. In addition, normal neovascularization was enhanced, and vessel network maturation was promoted in retina. In microscopic findings, the ganglion cells in inner layer of control group were bigger, and the retinal ganglion layer of mice treated with 10 µg/kg of EGT001 turned to normal, and the number of abnormal vessels was also significantly decreased. EGT001 appeared to induce ANG1 in blood. However, EGT001 did not induce the increasing of ANG1 in the liver, eye and spleen. Conclusions: These results showed that pro–angiogenic activity of EGT001. EGT001 could induce ANG1, then ANG1 helps damaged retinal vessel to mature vessel formation without inflammatory reactions. Therefore, EGT001 may be used as a therapeutic modulator for need of vessel maturation and stabilization on retinopathy of prematurity.
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