Purchase this article with an account.
S. Bakalian, J.P. Souza Filho, Z.M. Correa, J.–C.A. Marshall, A.B. Coutinho, M.N. Burnier, Jr; The Effect of Blue Light Exposure on the Proliferation Rate of Human Retinoblastoma Cell Lines . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4261.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: Studies have speculated on the role of sun exposure in the increased incidence of retinoblastoma in many countries. It has been suggested that in primates, blue light mediated reactive oxygen species (ROS) formation in the posterior segment of the eye. These ROS have been shown to be responsible for damage to retinal pigment epithelial cells, leading to cellular DNA damage. The aim of this study was to investigate the effect of blue light on the proliferation rates of two retinoblastoma cell lines. Methods: Two human retinoblastoma cell lines, Y79 and WERI–Rb–1, were utilized for this experiment. These cell lines were seeded at a concentration of 1x106 cells per well in a 24 well plate. Prior to exposure, the RPMI–1640 cell culture medium was removed and replaced with Hank’s buffered saline solution (HBSS). Cells were then exposed to blue light for 3 hours per day following which HBSS was then replaced with normal culture medium. This procedure was repeated for four days. Wells covered by aluminum foil were used as controls. The proliferation rate of the two–retinoblastoma cell lines compared to the controls was assessed using the MTT based proliferation assay. The Student’s t–test was used to compare the results for statistical significance. Results: The human retinoblastoma cell lines exposed to blue light showed a statistically significant increase in their proliferation compared to the control (p < 0.001). Exposure to blue light resulted in a two–fold increase in proliferation for both cell lines. Conclusions: The exposure of cells to blue light led to an increase in proliferation in both cell lines compared to the control. This increase in proliferation may be due to the formation of ROS induced by blue light. Therefore, we propose that these results should be taken into consideration in the possible use of blue light filtering intra–ocular lenses for children having cataract surgery.
This PDF is available to Subscribers Only